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园艺学报 ›› 2012, Vol. 39 ›› Issue (11): 2133-2141.

• 果树 • 上一篇    下一篇

利用SSR 标记进行枣树子代苗父本鉴定

王斯琪, 唐诗哲, 孔德仓, 贺润平, 刘华波, 麻丽颖, 刘君, 王哲, 李颖岳, 申连英, 庞晓明   

  1. (1 北京林业大学林木育种国家工程实验室,树木花卉遗传育种教育部重点实验室,计算生物学中心,生物科学与技术学院,北京 100083;2 河北省沧县国家枣树良种基地,河北沧州 061000;3 山西省农业科学院植物保护研究所,太原 030031;4 河北农业大学中国枣研究中心,河北保定 071001)
  • 出版日期:2012-11-25 发布日期:2012-11-25

Application of SSR Markers for the Identification of Paternal Parent for the Seedlings of Chinese Jujube

WANG  Si-Qi, TANG  Shi-Zhe, KONG  De-Cang, HE  Run-Ping, LIU  Hua-Bo, MA  Li-Ying, LIU  Jun, WANG  Zhe, LI  Ying-Yue, SHEN  Lian-Ying, PANG  Xiao-Ming   

  1. (1National Engineering Laboratory for Tree Breeding,Key Laboratory of Genetics and Breeding in Forest Trees and
    Ornamental Plants,Ministry of Education, Center for Computational Biology,College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,China;2National Key Forestry Station for Improved Jujube Cultivars,Cangzhou,Hebei 061001,China;3Institute of Plant Protection;Shanxi Academy of Agricultural Sciences,Taiyuan 030031,China;4Research center of Chinese Jujube,Agricultural University of Hebei,Baoding,Hebei 071001,China)
  • Online:2012-11-25 Published:2012-11-25

摘要: 利用SSR标记技术对‘冬枣’自然授粉实生苗1 728株和‘灵宝大枣’自然授粉实生苗530株进行父本鉴定,利用可能的父本材料从376对引物中筛选出8对条带清晰、多态性较高且具有高度区分度的引物。采用荧光M13毛细管电泳技术,用这8对引物在亲本材料中共检测到45个多态等位条带,每对引物的平均多态条带数为5.625,PIC值变幅为0.58 ~ 0.86,平均为0.71。利用Cervus3.0软件对具有8对引物数据的子代进行父本分析,鉴定出‘冬枣’ב木枣’实生苗399株、‘冬枣’ב小芽枣’实生苗390株、‘冬枣’ב映山红’实生苗357株、‘灵宝大枣’ב紫圆枣’(‘紫枣’)实生苗204株、‘灵宝大枣’ב柿饼枣’实生苗126株。

关键词: 枣, SSR, 父本鉴定, 毛细管电泳

Abstract: The paternalparents of 1 728 open-pollinated seedlings of‘Dongzao’(Ziziphus jujuba Mill.)and 530 open-pollinated seedlings of‘Lingbao Dazao’were identified with SSR technique. Eight primer pairs were selected from 376 primer pairs,which amplified distinct,polymorphic segments and showed high differential rate. Twelve candidate parents were analyzed by capillary electrophoresis using fluorescent M13 multi-state detection method. Totally,45 polymorphic alleles were revealed,with an average of 5.625 for each primer pairs. Polymorphism information content values for the primer pairs ranged from 0.58 to 0.86,with an average of 0.71. Using software Cervus3.0,399 hybrids from‘Dongzao’בMuzao’were identified,and 390 from‘Dongzao’בXiaoyazao’,357 from‘Dongzao’בYingshanhong’,204 from ‘Lingbao Dazao’בZiyuanzao(Zizao)’,126 from‘Lingbao Dazao’בShibingzao’were also identified.

Key words: jujube, SSR, paternal parent analysis, capillary electrophoresis

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