https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2012, Vol. 39 ›› Issue (9): 1849-1858.

• 综述 • 上一篇    下一篇

植物中的咖啡碱:从合成途径研究到转基因作物

 谭礼强, 齐桂年, 陈盛相, 王丽鸳, 韦 康, 成 浩   

  1. (1 四川农业大学园艺学院,四川雅安 614025;2 中国农业科学院茶叶研究所茶树改良中心,杭州 310008)
  • 出版日期:2012-09-25 发布日期:2012-09-25

Caffeine in Plants:From Biosynthesis Pathway to Genetically Modified

TAN  Li-Qiang, QI  Gui-Nian, CHEN  Sheng-Xiang, WANG  Li-Yuan, WEI   Kang, CHENG   Hao   

  1. (1College of Horticulture,Sichuan Agricultural University,Ya’an,Sichuan 614025,China;2National Center for Tea Improvement,Tea Research Institute,Chinese Academy of Agricultural Science,Hangzhou 310008,China)
  • Online:2012-09-25 Published:2012-09-25

摘要: 在植物细胞中咖啡碱的核心合成途径为:黄嘌呤核苷→7–甲基黄嘌呤核苷→7–甲基黄嘌呤→可可碱→咖啡碱,即3次顺序甲基化反应和1次核糖水解过程。目前已经从茶树等植物中分离出催化上述甲基化反应的酶,并克隆得到编码这些酶的基因。在此基础上,利用RNAi干扰和农杆菌介导法等技术,已成功培育出低咖啡碱饮料作物茶树和咖啡以及能合成咖啡碱的烟草和菊花植株。

关键词: 茶树, 咖啡植物, 咖啡碱, 合成途径, N–甲基转移酶, 转基因

Abstract: The main caffeine biosynthetic pathway is a sequence consisting of xanthosine → 7-methylxanthosine → 7-methylxanthine → theobromine → caffeine. In recent years,the enzymes and genes involved in caffeine biosynthesis have been isolated from Camellia and Coffea plants and genetically modified tea and coffee seedlings that producing low caffeine have been developed. Caffeine can also be synthesized in transgenic tobacco and chrysanthemum plants,which do not contain it naturally,for pests control purposes.

Key words: Camellia sinensis, Coffea, caffeine, biosynthesis, N-methyltransferase, genetically modified

中图分类号: