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园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1039-1046.

• 观赏植物 • 上一篇    下一篇

创伤及高渗透压预处理对蝴蝶兰叶片诱导类原球茎的影响

黄 磊1,2;陈之林1;吴坤林1;曾宋君1;段 俊1*
  

  1. 1中国科学院华南植物园,广州510650;2中国科学院研究生院,北京100049)
  • 收稿日期:2008-03-17 修回日期:2008-05-22 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 段 俊

Effects of wound and high osmotic pressure pretreatments on PLB induction from leaf explants in Phalaenopsis amabilis

HUANG Lei1,2,CHEN Zhi-lin1,Wu Kun-ling1,ZENG Song-jun1,and DUAN Jun1*   

  1. 1South China Botanical Garden,the Chinese Academy of Sciences,Guangzhou 510650,China;2the Graduate School of the Chinese Academy of Sciences,Beijing 100049,China)
  • Received:2008-03-17 Revised:2008-05-22 Online:2008-07-25 Published:2008-07-25
  • Contact: DUAN Jun

摘要:

以蝴蝶兰试管播种苗的顶端第1叶和第2叶叶片为外植体,以1/2 MS + 10% 椰子乳 + NAA 0.1 mg·L-1 + TDZ 1.0 mg·L-1 + 0.5% 琼脂为诱导培养基,研究了创伤和高渗透压两类预处理对其诱导类原球茎(protocorm-like body,PLB)的影响。结果表明,两类预处理均对叶片诱导PLB有显著促进作用。在创伤预处理中,当外植体横切2刀及用刀尖扎15次后,外植体的PLB诱导率分别达到66.1%和68.3%,平均每外植体诱导出13.1和15.2个PLB;在高渗透压预处理中,当用0.4 mol·L-1甘露醇溶液和AAM培养基分别浸泡2 h后,外植体的PLB诱导率分别达到55.2%和51.3%,平均每个外植体诱导出13.7和12.9个PLB,而对照的PLB诱导率只有19.5%,平均每个外植体只诱导出3.1个PLB;经上述两类预处理后外植体的PLB诱导率和平均PLB个数均与对照达极显著差异水平,同时,外植体上诱导出PLB的时间分别比对照提前了10 d和5 d。但来源于第1叶和第2叶的外植体之间的PLB诱导结果均没有明显差异。经创伤和高渗透压预处理后诱导出的PLB均能正常分化成苗。

关键词: 蝴蝶兰, 叶片, 预处理, PLB诱导

Abstract:

The first and second leaf from the top of Phalaenopsis amabilis seedlings were used respectively as explants,and 1/2 MS medium including 10% Coconut Milk (CM), 0.1 mg·L-1 α-Naphthyl Acetic Acid (NAA), 1.0 mg·L-1 Thidiazuron (TDZ) and 0.5% agar was used to induce PLB, the effects of wound and high osmotic pressure on PLB(protocorm-like body)induction were studied. The results revealed that these two types of pretreatments could improve PLB induction remarkably. After the explants were cut transversely twice or stabbed 15 times on different sites, their PLB induction rates went up to 66.1% or 68.3%, the PLB number per explant went up to13.1 or 15.2. After the explants were incubated in 0.4 mg·L-1 mannitol solution or AAM medium for 2 hours as high osmotic pressure pretreatments, the PLB induction rates went up to 55.2% or 51.3%, the PLB number per explant went up to 13.7 or 12.9. While the PLB induction rate of the control was 19.5% and the PLB number per explant was 3.1. Compared with the control, the PLB induction rate and PLB number per explant were both significantly higher in all the pretreatments. Moreover, after the explants were pretreated by wound or high osmotic pressure, the time of PLB formation was 10 or 5 days shorter respectively than that of the control. However, there were not obvious differences between the first and second leaf explants in PLB induction. PLB induced from the explants that had been pretreated by wound or high osmotic pressure could develop into plantlet normally.

Key words: Phalaenopsis, Leaf, Pretreatment, PLB induction

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