园艺学报 ›› 2003, Vol. 30 ›› Issue (6): 663-667.

• 研究论文 • 上一篇    下一篇


张鲁刚; 惠麦侠; 张明科   

  1. (西北农林科技大学园艺学院,杨凌 712100)
  • 收稿日期:2003-01-27 修回日期:2003-04-08 出版日期:2003-12-25 发布日期:2003-12-25

Vigor and Hybridizing Ability of Trace Pollen of Temperature-sensitive CMS in Chinese Cabbage

Zhang Lugang; Hui Maixia; Zhang Mingke

  1. (College of Horticulture,Northwest Sci-tech University of Agriculture &Forestry, Yangling 712100, China)
  • Received:2003-01-27 Revised:2003-04-08 Online:2003-12-25 Published:2003-12-25

摘要: 以大白菜[Brassica campestris L.ssp.peldnensis(Lour.) Olsson]的欧洲油菜(Brassica napus L.)胞质雄性不育系CMS3411-7和CMS7311为试材,通过人工低温诱导产生微量花粉,研究了不同育性级别花药中的相对正常花粉率以及1~2级花药中花粉的相对生活力和杂交竞争力。结果表明:诱导产生的1、2、3级花药中的花粉量分别相当于正常花药的73.5% ~84.4% 、47.6% ~51.5% (半不育)和28.4% ~32.5% ,4级花药中均无花粉;1、2级花药中的花粉具有授粉能力, 自交单荚结籽3.9粒,相当于低自交不
亲和;在提前4 h授粉的情况下,1、2级花药中花粉的杂交竞争力仅是紫菜薹(Brassica campestris L.ssp.chinensis L.var.purpurea Bailey)正常花粉的9.1% ~16.6% ,即正常花粉的1/6~1/10。最后提出了利用温度敏感胞质雄性不育制杂交种时应注意的问题,讨论了利用人工诱导微量花粉进行不育系自身保持的可行性.

关键词: 大白菜, 温度敏感雄性不育, 花粉, 生活力, 杂交竞争力

Abstract: The male sterile line of Chinese cabbage CMS3411-7 and CMS7311, with cytoplasm of Brassica campestris L.were induced under 5 /9 days condition to obtain trace pollens, which relative vigor an d cross-fertile abihty was tested by biological way. Its results show that the quan tity of trace po len in an ther of sterile grade 1,grade 2 and ade 3 were 73.5%-84.4% ,47.6%-51.5% (half sterile)and 28.4%-32.5% of normal anther respectively; the trace pollen in an ther of sterile grade 1 and grade 2 both Can fertile by self-polinated and got about 3.9 grain seeds per silique, which is similar to the situation of low incompatible line; the cross-fertile ability of it is only 9.1%-6.6% compared with po llen of flowering Chinese cab bage (Brassica campestris L. ssp. chinensis L.var.purpurea Bailey) even pollinated 4 h before.This is about one sixth to one tenth of normal pollen.Additional, we present the qu estion that should be paid attention to in hybrid production, and the reliab ility of using the trace pollen induced to reproduce CMS lines was discussed.

Key words: Chinese cab bage, Tempe rature-sensitive CMS, Vigor of pollen, Cross·fertile ability