园艺学报 ›› 2004, Vol. 31 ›› Issue (2): 249-252.

• 研究报告 • 上一篇    下一篇


陈 超;王桂兰;田立民;崔瑞生

  1. (唐山师范学院生物科学技术系,唐山063000)
  • 收稿日期:2003-06-23 修回日期:2003-10-08 出版日期:2004-04-25 发布日期:2004-04-25

Embryoid Induction and Regeneration in Callus of Kalanchoe blossfeldiana

Chen Chao;Wang Guilan;Tian Limin;Cui Ruisheng


  1. (Department of Biological Science and Technology,Tangshan Teacher's College,Tangshan 063000,China)
  • Received:2003-06-23 Revised:2003-10-08 Online:2004-04-25 Published:2004-04-25

摘要: 研究了长寿花胚性愈伤组织的筛选,胚状体的诱导发生、发育过程及植株再生。经过对外观
及细胞学观察,看出外观质地疏松、颗粒状的淡黄色愈伤组织细胞圆形且形状规则,是胚性愈伤组织。诱导胚性愈伤组织的最适培养基为:MS+2,4-D 2 mg·L-1 +BA 0.2 mg·L-1;胚状体的诱导培养基为MS+BA 2 mg·L-1 +NAA 0.2 mg·L-1 +活性炭4 g·L-1+蔗糖30 g·L-1,胚状体诱导率可达185个胚状体;胚状体的再生培养基为MS+蔗糖30 g·L-1。利用石蜡切片对胚状体的发生过程进行了观察。

关键词: 长寿花, 胚性愈伤组织, 胚状体, 再生

Abstract: The study aimed on the selection of embryonic callus of Kalanchoe blossbeldiana,the induction of embryoid,morphogenesis of embryoid and regeneration.The grain-like,light yellow and loose callus was embryonic callus. During the inducing of embryoid,the culture mediums with different growth regulator concentration were used.Finally the culture medium with the highest of reduction frequency was MS+BA 2 mg·L-1+NAA 0.2 mg·L-1+Sucrose 30 g·L-1 +Active carbon 4 g·L-1 (185 embryoid per gram).The regeneration medium Was MS+Sucrose 30 g·L-1.The appearance and growing of embryoid were also observed through parafin slices of callus.The development procedure of embryoid Was procel of embryoid,globular stage,heart-shape stage and cotyledonary stage

Key words: Kalanchoe blossfeldiana, Embryonic callus, Embryoid, Regeneration