关键词: 天南星科, 白鹤芋, 胚性愈伤组织, 液体培养, 再生

Abstract: This study established embryogenic cell suspension cultures of Spathiphyllum cannifolium through embryogenic calli induced from the petioles of plantlets in vitro，and further achieved high frequency of plant regeneration using the obtained suspension cultures. The results suggested that the optimal conditions of embryogenic cell suspension culture included：Inoculum amount was 0.3 g of embryogenic cell aggregates loaded with 20 mL fluid nutrient medium；Suspension culture medium was MS medium supplemented with 0.5 mg ? L-1 N-phenyl-N’-1,2,3-thiadiazol-5-ylurea（TDZ），1.0 mg ? L-1 2,4- Dichlorophenoxyacetic acid（2,4-D），and 30 g ? L-1 sucrose or maltose（pH 5.8）；Subculture interval was 14 days. Under these circumstances，the proliferation rate of embryogenic cell aggregates was 5-fold per subculture cycle. For plant regeneration，moreover，25.1 plantlets in average developed（or generated）from each embryogenic cell aggregate on the optimal differential medium of 1/2MS + 0.3 mg ? L-1 6-BA + 30 g ? L-1 sucrose + 8.0 g ? L-1agar（pH 5.8）. The status of rapid proliferation of embryogenic cells andefficient regeneration of plantlets could maintain 24 weeks.

Key words: Araceae, Spathiphyllum cannifolium, embryogenic calli, submerged culture, regeneration