甜瓜GolS家族基因鉴定及其响应低温胁迫的表达分析

doi:10.16420/j.issn.0513-353x.2024-0111

摘要/Abstract

摘要：

为探索甜瓜肌醇半乳糖苷合成酶（GolS）基因的特性及其响应低温胁迫的表达模式，利用生物信息手段对其家族成员进行全基因组鉴定及染色体位置、进化关系、基因结构、启动子顺式作用元件、组织特异性表达和在低温胁迫下的表达模式等进行分析。结果鉴定出12个CmGolS不均匀地分布在9条染色体上，并且在系统发育树中被分为5族。理化性质分析表明甜瓜CmGolS蛋白均为亲水性蛋白。基因结构分析表明CmGolS基因保守性较高，多有2 ~ 5个内含子且含有与胁迫和赤霉素、生长素、脱落酸等激素响应相关的顺式作用元件以及保守基序“DxD”和“HxxGxxKPW”。CmGolS具有组织表达特异性，大多在雄花中表达，并且在低温胁迫处理下相对表达量大多呈显著上调趋势，表明CmGolS参与响应低温胁迫。

关键词: 甜瓜, 肌醇半乳糖苷合成酶, 生物信息分析, 低温胁迫, 表达分析

Abstract:

In order to study the characteristics of melon（Cucumis melo L.）GolS family genes and their responses to low temperature stress，members of the melon GolS family of genes were identified using bioinformatics. The chromosomal locations，evolutionary relationships，genetic structure，promoters，cis-acting elements，tissue-specific expressions and expression patterns under low temperature stress were predicted using bioinformatics. After exhaustive analysis，12 CmGolS genes were successfully identified and found to be unevenly distributed across nine chromosomes. The analyses of phylogenetic showed that these genes were divided into 5 groups. Physicochemical analysis showed that all CmGolS proteins were hydrophilic proteins. The CmGolS genes were highly conserved and they have 2-5 introns. These genes contain conserved motifs“DxD”“HxxGxxKPW”and many cis-acting elements，which related to stress and hormones such as gibberellin，auxin and abscisic acid. In addition CmGolS genes have tissue specific expressions，most of them are expressed in male flowers. And the relative expression of most of them show a significant up-regulation trend under low temperature stress that indicated CmGolS genes can actively participate in the processes of responding to low temperature stress.

Key words: melon, galactinol synthase, bioinformatics analysis, cold stress, expression analysis

贺丹丹, 何宏泰, 王文庭, 周文美, 刘燕敏, 刘骕骦. 甜瓜GolS家族基因鉴定及其响应低温胁迫的表达分析[J]. 园艺学报, 2025, 52(1): 136-148.

HE Dandan, HE Hongtai, WANG Wenting, ZHOU Wenmei, LIU Yanmin, LIU Sushuang. Identification of Melon GolS Genes Family and the Expression Analysis in Response to Low Temperature Stress[J]. Acta Horticulturae Sinica, 2025, 52(1): 136-148.

图/表 8

参考文献 41

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基因 Gene	引物 Primer	序列（5′-3′） Sequence	大小/bp Size
CmGolS1	MELO3C015870.2.1	F2：GGAATCATCCGAAAACCTACCT；R2：ACACGAGAACACCTCCCCTT	234
CmGolS3	MELO3C010862.2.1	F2：CGAGAGGGAGATTGGTGGTT；R2：AGGAAGTTTGTTCGTCGTGGT	689
CmGolS4	MELO3C012925.2.1-F3	F3：GTCACCTCTCCCACTCCCTTT；R3：TTTGCATGTTCTCTTCTTTCCC	211
CmGolS5	MELO3C008614.2.1-F1	F1：AATTCTGGTGTTATGGTTGTGGA；R1：CTTCTTGTGGTAAGTTTGGCTCA	166
CmGolS7	MELO3C008045.2.1-F2	F2：TCAGGAACCCAAAAGCAGAAA；R2：GCGGCGTAGGTACGTAAAACT	783
CmGolS8	MELO3C022024.2.1	F1：GCCATTCGTCTTCATTCTCACT；R1：TTGGACTCCCTTATCTCCTTCTC	315
CmEFIa	CmEFIa	F：ACTGTGCTGTCCTCATTATTG；R：AGGGTGAAAGCAAGAAGAGC	98

基因 Gene	引物 Primer	序列（5′-3′） Sequence	大小/bp Size
CmGolS1	MELO3C015870.2.1	F2：GGAATCATCCGAAAACCTACCT；R2：ACACGAGAACACCTCCCCTT	234
CmGolS3	MELO3C010862.2.1	F2：CGAGAGGGAGATTGGTGGTT；R2：AGGAAGTTTGTTCGTCGTGGT	689
CmGolS4	MELO3C012925.2.1-F3	F3：GTCACCTCTCCCACTCCCTTT；R3：TTTGCATGTTCTCTTCTTTCCC	211
CmGolS5	MELO3C008614.2.1-F1	F1：AATTCTGGTGTTATGGTTGTGGA；R1：CTTCTTGTGGTAAGTTTGGCTCA	166
CmGolS7	MELO3C008045.2.1-F2	F2：TCAGGAACCCAAAAGCAGAAA；R2：GCGGCGTAGGTACGTAAAACT	783
CmGolS8	MELO3C022024.2.1	F1：GCCATTCGTCTTCATTCTCACT；R1：TTGGACTCCCTTATCTCCTTCTC	315
CmEFIa	CmEFIa	F：ACTGTGCTGTCCTCATTATTG；R：AGGGTGAAAGCAAGAAGAGC	98

名称 Name	ID	氨基酸数 Animo acid number	分子质量/kD	等电点pI	不稳定系数Coefficient of instability	平均亲水指数 Grand average of hydropathicity	亚细胞定位 Prediction of subcellular localization
CmGolS1	MELO3C015870.2.1	367	42.13	8.70	41.22	-0.274	叶绿体Chloroplast
CmGolS2	MELO3C008407.2.1	655	76.19	6.64	45.07	-0.368	叶绿体Chloroplast
CmGolS3	MELO3C010862.2.1	520	61.73	8.18	53.33	-0.482	细胞质Cytoplasm
CmGolS4	MELO3C012925.2.1	335	38.51	4.97	44.32	-0.291	细胞质Cytoplasm
CmGolS5	MELO3C008614.2.1	414	46.95	6.53	40.70	-0.033	质膜Plasma membrane
CmGolS6	MELO3C004270.2.1	614	71.32	8.32	57.16	-0.332	叶绿体Chloroplast
CmGolS7	MELO3C008045.2.1	567	65.91	6.37	40.38	-0.351	细胞质Cytoplasm
CmGolS8	MELO3C022024.2.1	534	61.53	8.53	43.13	-0.179	液泡Vacuole
CmGolS9	MELO3C012034.2.1	377	42.76	6.86	52.29	-0.156	液泡Vacuole
CmGolS10	MELO3C011991.2.1	332	38.00	5.47	40.36	-0.241	细胞质Cytoplasm
CmGolS11	MELO3C021249.2.1	331	38.29	4.78	42.58	-0.303	细胞质Cytoplasm
CmGolS12	MELO3C025521.2.1	325	37.79	5.60	40.54	-0.343	细胞质Cytoplasm

名称 Name	ID	氨基酸数 Animo acid number	分子质量/kD	等电点pI	不稳定系数Coefficient of instability	平均亲水指数 Grand average of hydropathicity	亚细胞定位 Prediction of subcellular localization
CmGolS1	MELO3C015870.2.1	367	42.13	8.70	41.22	-0.274	叶绿体Chloroplast
CmGolS2	MELO3C008407.2.1	655	76.19	6.64	45.07	-0.368	叶绿体Chloroplast
CmGolS3	MELO3C010862.2.1	520	61.73	8.18	53.33	-0.482	细胞质Cytoplasm
CmGolS4	MELO3C012925.2.1	335	38.51	4.97	44.32	-0.291	细胞质Cytoplasm
CmGolS5	MELO3C008614.2.1	414	46.95	6.53	40.70	-0.033	质膜Plasma membrane
CmGolS6	MELO3C004270.2.1	614	71.32	8.32	57.16	-0.332	叶绿体Chloroplast
CmGolS7	MELO3C008045.2.1	567	65.91	6.37	40.38	-0.351	细胞质Cytoplasm
CmGolS8	MELO3C022024.2.1	534	61.53	8.53	43.13	-0.179	液泡Vacuole
CmGolS9	MELO3C012034.2.1	377	42.76	6.86	52.29	-0.156	液泡Vacuole
CmGolS10	MELO3C011991.2.1	332	38.00	5.47	40.36	-0.241	细胞质Cytoplasm
CmGolS11	MELO3C021249.2.1	331	38.29	4.78	42.58	-0.303	细胞质Cytoplasm
CmGolS12	MELO3C025521.2.1	325	37.79	5.60	40.54	-0.343	细胞质Cytoplasm