茄子绿果基因的精细定位及其KASP标记开发

doi:10.16420/j.issn.0513-353x.2023-0567

园艺学报 ›› 2024, Vol. 51 ›› Issue (9): 2008-2018.doi: 10.16420/j.issn.0513-353x.2023-0567

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

茄子绿果基因的精细定位及其KASP标记开发

夏志磊¹, 俞冰昕¹, 杨梦¹, 连子林¹, 官利兰², 何艺超², 颜爽爽¹, 曹必好¹, 邱正坤¹^,^*()

¹ 华南农业大学园艺学院，农业农村部华南地区园艺作物生物学与种质创制重点实验室，广州 510642
² 广东省恩平市农业技术推广服务中心，广东江门 529499

收稿日期:2024-05-17 修回日期:2024-07-16 出版日期:2024-09-25 发布日期:2024-09-19
通讯作者:
^* E-mail：qiuzhengkun@scau.edu.cn
基金资助:
广东省基础与应用基础研究基金项目(2023A1515030070); 广东省基础与应用基础研究基金项目(2022A1515010734); 广东省重点研发计划项目(2022B020208003); 广东省种业振兴项目(2022-NPY-00-026); 广东省现代农业产业技术果菜产业体系创新团队项目(2023KJ110); 海南省重点研发项目(ZDYF2023XDNY041)

Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant

XIA Zhilei¹, YU Bingxin¹, YANG Meng¹, LIAN Zilin¹, GUAN Lilan², HE Yichao², YAN Shuangshuang¹, CAO Bihao¹, QIU Zhengkun¹^,^*()

¹ Key Laboratory of Biology and Genetic Improvement of Horticultural Crops，Ministry of Agriculture and Rural Affairs，College of Horticulture，South China Agricultural University，Guangzhou 510642，China
² Guangdong Enping Agricultural Technology Extension Center，Jiangmen，Guangdong 529499，China

Received:2024-05-17 Revised:2024-07-16 Published:2024-09-25 Online:2024-09-19

摘要/Abstract

摘要：

以白茄高代自交系E802与绿茄高代自交系NY为亲本构建的F₂群体为试验材料，对茄子果色基因进行遗传连锁分析。结果显示，在304株F₂群体中绿茄与白茄单株数符合3︰1孟德尔单基因分离比，其F₁植株果实为绿色，表明绿茄对白茄为显性，受一个主效基因控制。以F₂及其F_2:3家系为材料，采用BSA-seq结合KASP分子标记连锁分析，将绿茄基因精细定位于8号染色体末端约683.4 kb的范围内，其遗传距离约为0.17 cM。根据茄子基因组注释信息，该区间内含有46个基因。结合序列分析、同源分析将SmAPRR2作为绿茄关键候选基因。SmAPRR2在白茄E802中存在5.27 kb的大片段缺失，使其缺失第7 ~ 11号外显子，导致其功能缺失。进一步开发了与SmAPRR2紧密连锁的KASP分子标记22QP296，其在F₂分离群体中的基因型和表型一致率为100%，在194份茄子自然材料中基因型和表型一致率为94.85%，可作为茄子果色分子育种的分子标记。

关键词: 茄子, 果实颜色, 基因定位, KASP标记, 绿果基因, APRR2

Abstract:

An F₂ population developing by a white fruit eggplant line E802 and a green fruit eggplant line NY was used for the genetic analysis of the gene underlying green fruit color. The segregation ratio of green fruit and white in the 304 individuals in the F₂ population was 3︰1，indicating that the green phenotype of NY is controlled by a single dominant locus. Combing with the BSA-seq and linkage-based QTL mapping，the gene underlying green fruit color of eggplant was fine-mapped into 683.4 kb interval on chromosome 8 using F₂ and the F_2:3population. The genetic distance of this locus is 0.17 cM. Among the 46 genes in the interval，SmAPRR2 was the best candidate gene. The 5.27 kb deletion was detected in the E802，resulting the SmAPRR2 lacking the exon 7 to 11. In addition，a tightly linked to SmAPRR2 KASP marker，22QP296，was development. The accuracies of 22QP296 for predicting the skin color（green or white）in the F₂ population and the 194 cultivars were 100% and 94.85%，respectively，indicating that this KASP marker can be used in molecular marker-assisted selection in eggplant breeding.

Key words: eggplant, fruit color, gene mapping, KASP marker, green fruit gene, APRR2

夏志磊, 俞冰昕, 杨梦, 连子林, 官利兰, 何艺超, 颜爽爽, 曹必好, 邱正坤. 茄子绿果基因的精细定位及其KASP标记开发[J]. 园艺学报, 2024, 51(9): 2008-2018.

XIA Zhilei, YU Bingxin, YANG Meng, LIAN Zilin, GUAN Lilan, HE Yichao, YAN Shuangshuang, CAO Bihao, QIU Zhengkun. Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant[J]. Acta Horticulturae Sinica, 2024, 51(9): 2008-2018.

图/表 6

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引物名称 Primer name	引物序列（5′-3′） Primer sequence
22QP296	F_P1：GAAGGTGACCAAGTTCATGCTAAGGACAGTCTAATCACCACTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTAAGGACAGTCTAATCACCACTCTA
	R：GCATTTGGGAGACATGCATGAATA
22QP299	F_P1：GAAGGTGACCAAGTTCATGCTAATTGCCGTTCAAAGTGTACGTAC
	F_P2：GAAGGTCGGAGTCAACGGATTAATTGCCGTTCAAAGTGTACGTAT
	R：AAACTGGTCGAACAATAAAGGACG
22QP302	F_P1：GAAGGTGACCAAGTTCATGCTAGGAATATCTTCTCTCGTCTCGAC
	F_P2：GAAGGTCGGAGTCAACGGATTAGGAATATCTTCTCTCGTCTCGAT
	R：AATTGTACAACATACCCTCCAGCA
22QP308	F_P1：GAAGGTGACCAAGTTCATGCTGGGAAGTGCATTCTACTTTTCATACA
	F_P2：GAAGGTCGGAGTCAACGGATTGGGAAGTGCATTCTACTTTTCATACT
	R：GTTGCTCCGCTCTTAATCAAAAGT
22QP311	F_P1：GAAGGTGACCAAGTTCATGCTACATAATTCCTCGATTAAATGTAGGGAATA
	F_P2：GAAGGTCGGAGTCAACGGATTACATAATTCCTCGATTAAATGTAGGGAATG
	R：TCTTTGTCACGTCATACTAATTGCA
22QP314	F_P1：GAAGGTGACCAAGTTCATGCTTGTTTGGTATGAATATAAAGGACCACTAAT
	F_P2：GAAGGTCGGAGTCAACGGATTTGTTTGGTATGAATATAAAGGACCACTAAC
	R：TGAATTCATCACGGACACAATTGT
22QP317	F_P1：GAAGGTGACCAAGTTCATGCTGGGAGAACGACACAAGCTGA
	F_P2：GAAGGTCGGAGTCAACGGATTGGGAGAACGACACAAGCTGT
	R：ACCTGAATGCACATGATAGTGACT
22QP320	F_P1：GAAGGTGACCAAGTTCATGCTTGAGTAAACTAACTTCTAGTCTCTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTTGAGTAAACTAACTTCTAGTCTCTCTA
	R：TGGCACACAAATTAATACTCCAGA
22QP323	F_P1：GAAGGTGACCAAGTTCATGCTTGTTGTATATCGAGCATTGGTTGATTAA
	F_P2：GAAGGTCGGAGTCAACGGATTTGTTGTATATCGAGCATTGGTTGATTAG
	R：GAACTTTAAAACACAGTCAGCGGT
22QP326	F_P1：GAAGGTGACCAAGTTCATGCTCTTACTCTACGTCCCAAAGTCCCT
	F_P2：GAAGGTCGGAGTCAACGGATTCTTACTCTACGTCCCAAAGTCCCC
	R：ACAAAACCGCTAAATCTACCCCTA
22QP437	F_P1：GAAGGTGACCAAGTTCATGCTATTACTGAGTTGCTCATCCATGAA
	F_P2：GAAGGTCGGAGTCAACGGATTATTACTGAGTTGCTCATCCATGAT
	R：ACCTTCACCATCTTGTACTTGAGA
22QP539	F_P1：GAAGGTGACCAAGTTCATGCTACTCTTCAGTGCTCAGTTCCATTAT
	F_P2：GAAGGTCGGAGTCAACGGATTACTCTTCAGTGCTCAGTTCCATTAG
	R：AACAAAAACCATTGCCAGAGTGAG
22QP542	F_P1：GAAGGTGACCAAGTTCATGCTGGTGTTAAGATCAATATAATTAGGCTCCAAT
	F_P2：GAAGGTCGGAGTCAACGGATTGGTGTTAAGATCAATATAATTAGGCTCCAAA
	R：GGAAAAGTGGAGAAAAGCACGAAA
22QP545	F_P1：GAAGGTGACCAAGTTCATGCTAGAATATCTGATTCGAGTTTAATTAAAACCA
	F_P2：GAAGGTCGGAGTCAACGGATTAGAATATCTGATTCGAGTTTAATTAAAACCT
	R：TTCCATCACTTGTTGTTAGCTTCT
22QP548	F_P1：GAAGGTGACCAAGTTCATGCTTCTTACTTGTTAATTACGTCTTTCTATTCAG
	F_P2：GAAGGTCGGAGTCAACGGATTTCTTACTTGTTAATTACGTCTTTCTATTCAA
	R：TGGATGGAATTTCTTTGTCCAAAA
23QP26	F_P1：GAAGGTGACCAAGTTCATGCTATTTATCCATATTTATCACGAGTTCATATAAG
	F_P2：GAAGGTCGGAGTCAACGGATTATTTATCCATATTTATCACGAGTTCATATAAA
	R：GCCGAGTTCAAACTTCAAAGTACT
23QP29	F_P1：GAAGGTGACCAAGTTCATGCTTCCAGGAGGGAATGTTTTCGC
	F_P2：GAAGGTCGGAGTCAACGGATTTCCAGGAGGGAATGTTTTCGT
	R：TTAGTCGGAAGCCTCTCAATTTCA
23QP32	F_P1：GAAGGTGACCAAGTTCATGCTCTTTGCCTCCTTTTGTTGTTTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTCTTTGCCTCCTTTTGTTGTTTCTA
	R：GGTCATACTCTACCGTCCTTTTGT

引物名称 Primer name	引物序列（5′-3′） Primer sequence
22QP296	F_P1：GAAGGTGACCAAGTTCATGCTAAGGACAGTCTAATCACCACTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTAAGGACAGTCTAATCACCACTCTA
	R：GCATTTGGGAGACATGCATGAATA
22QP299	F_P1：GAAGGTGACCAAGTTCATGCTAATTGCCGTTCAAAGTGTACGTAC
	F_P2：GAAGGTCGGAGTCAACGGATTAATTGCCGTTCAAAGTGTACGTAT
	R：AAACTGGTCGAACAATAAAGGACG
22QP302	F_P1：GAAGGTGACCAAGTTCATGCTAGGAATATCTTCTCTCGTCTCGAC
	F_P2：GAAGGTCGGAGTCAACGGATTAGGAATATCTTCTCTCGTCTCGAT
	R：AATTGTACAACATACCCTCCAGCA
22QP308	F_P1：GAAGGTGACCAAGTTCATGCTGGGAAGTGCATTCTACTTTTCATACA
	F_P2：GAAGGTCGGAGTCAACGGATTGGGAAGTGCATTCTACTTTTCATACT
	R：GTTGCTCCGCTCTTAATCAAAAGT
22QP311	F_P1：GAAGGTGACCAAGTTCATGCTACATAATTCCTCGATTAAATGTAGGGAATA
	F_P2：GAAGGTCGGAGTCAACGGATTACATAATTCCTCGATTAAATGTAGGGAATG
	R：TCTTTGTCACGTCATACTAATTGCA
22QP314	F_P1：GAAGGTGACCAAGTTCATGCTTGTTTGGTATGAATATAAAGGACCACTAAT
	F_P2：GAAGGTCGGAGTCAACGGATTTGTTTGGTATGAATATAAAGGACCACTAAC
	R：TGAATTCATCACGGACACAATTGT
22QP317	F_P1：GAAGGTGACCAAGTTCATGCTGGGAGAACGACACAAGCTGA
	F_P2：GAAGGTCGGAGTCAACGGATTGGGAGAACGACACAAGCTGT
	R：ACCTGAATGCACATGATAGTGACT
22QP320	F_P1：GAAGGTGACCAAGTTCATGCTTGAGTAAACTAACTTCTAGTCTCTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTTGAGTAAACTAACTTCTAGTCTCTCTA
	R：TGGCACACAAATTAATACTCCAGA
22QP323	F_P1：GAAGGTGACCAAGTTCATGCTTGTTGTATATCGAGCATTGGTTGATTAA
	F_P2：GAAGGTCGGAGTCAACGGATTTGTTGTATATCGAGCATTGGTTGATTAG
	R：GAACTTTAAAACACAGTCAGCGGT
22QP326	F_P1：GAAGGTGACCAAGTTCATGCTCTTACTCTACGTCCCAAAGTCCCT
	F_P2：GAAGGTCGGAGTCAACGGATTCTTACTCTACGTCCCAAAGTCCCC
	R：ACAAAACCGCTAAATCTACCCCTA
22QP437	F_P1：GAAGGTGACCAAGTTCATGCTATTACTGAGTTGCTCATCCATGAA
	F_P2：GAAGGTCGGAGTCAACGGATTATTACTGAGTTGCTCATCCATGAT
	R：ACCTTCACCATCTTGTACTTGAGA
22QP539	F_P1：GAAGGTGACCAAGTTCATGCTACTCTTCAGTGCTCAGTTCCATTAT
	F_P2：GAAGGTCGGAGTCAACGGATTACTCTTCAGTGCTCAGTTCCATTAG
	R：AACAAAAACCATTGCCAGAGTGAG
22QP542	F_P1：GAAGGTGACCAAGTTCATGCTGGTGTTAAGATCAATATAATTAGGCTCCAAT
	F_P2：GAAGGTCGGAGTCAACGGATTGGTGTTAAGATCAATATAATTAGGCTCCAAA
	R：GGAAAAGTGGAGAAAAGCACGAAA
22QP545	F_P1：GAAGGTGACCAAGTTCATGCTAGAATATCTGATTCGAGTTTAATTAAAACCA
	F_P2：GAAGGTCGGAGTCAACGGATTAGAATATCTGATTCGAGTTTAATTAAAACCT
	R：TTCCATCACTTGTTGTTAGCTTCT
22QP548	F_P1：GAAGGTGACCAAGTTCATGCTTCTTACTTGTTAATTACGTCTTTCTATTCAG
	F_P2：GAAGGTCGGAGTCAACGGATTTCTTACTTGTTAATTACGTCTTTCTATTCAA
	R：TGGATGGAATTTCTTTGTCCAAAA
23QP26	F_P1：GAAGGTGACCAAGTTCATGCTATTTATCCATATTTATCACGAGTTCATATAAG
	F_P2：GAAGGTCGGAGTCAACGGATTATTTATCCATATTTATCACGAGTTCATATAAA
	R：GCCGAGTTCAAACTTCAAAGTACT
23QP29	F_P1：GAAGGTGACCAAGTTCATGCTTCCAGGAGGGAATGTTTTCGC
	F_P2：GAAGGTCGGAGTCAACGGATTTCCAGGAGGGAATGTTTTCGT
	R：TTAGTCGGAAGCCTCTCAATTTCA
23QP32	F_P1：GAAGGTGACCAAGTTCATGCTCTTTGCCTCCTTTTGTTGTTTCTG
	F_P2：GAAGGTCGGAGTCAACGGATTCTTTGCCTCCTTTTGTTGTTTCTA
	R：GGTCATACTCTACCGTCCTTTTGT

表型 Phenotype	基因型 Genotype	一致 Correct	不一致 Incorrect	正确率/% Accuracy rate
绿茄 Green fruit	杂合Heterozygous	50	1	94.85
	NY	131	5	94.85
白茄 White fruit	E802	3	4

表型 Phenotype	基因型 Genotype	一致 Correct	不一致 Incorrect	正确率/% Accuracy rate
绿茄 Green fruit	杂合Heterozygous	50	1	94.85
	NY	131	5	94.85
白茄 White fruit	E802	3	4

茄子绿果基因的精细定位及其KASP标记开发

Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant

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茄子绿果基因的精细定位及其KASP标记开发

Fine-Mapping and KASP Marker Development for the Gene Underlying Green Fruit in Eggplant

RichHTML

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摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 34

相关文章 15

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