关键词: 辣椒, 辣椒叶脉黄化病毒, P0基因, CP基因, MP基因

Abstract: In order to find out the occurrence and distribution of pepper vein yellows virus（PeVYV）and its molecular variation on pepper in Guizhou. 548 suspected samples from 25 sampling location in Guizhou Province were analyzed using RT-PCR. The results showed that 129 samples were positive and the detection rate was 23.54%. The sequence alignment of the PeVYV isolates from 14 different locations with the reported PeVYV isolates from China and other countries was performed，the nucleotide and amino acid homology of P0 gene was 90.9%–100.0%，amino acid similarity was 97.7%–100.0%，the nucleotide and amino acid homology of CP gene was 93.7%–100.0%，amino acid similarity was 97.9%–100.0%，the nucleotide and amino acid homology of MP gene was 94.7%–100.0%，amino acid similarity was 100.0%. In the phylogenetic tree，the results indicated that P0 of PeVYV from 13 locations were clustered and closely related to the isolates（KP326573），isolates from Bozhou，Zunyi，were closely related to the isolates from Australia. All PeVYV isolates CP gene in Guizhou were clustered with isolates from China，Australia，the United States and Spain. But the PeVYV isolates from different locations were clustered into two different branches. All MP genes from different locations were clustered together，and there was no significant difference in comparing with isolates from the other regions. The research results show that PeVYV isolates from different regions had molecular variation and genetic differentiation.

Key words: pepper, pepper vein yellow virus, P0 gene, CP gene, MP gene