利用宏基因组测序分析华南地区香蕉线条病毒多样性

doi:10.16420/j.issn.0513-353x.2024-0108

摘要/Abstract

摘要：

通过宏基因组测序对为害中国华南地区香蕉的病毒种类进行分析，发现16种病毒，大多数属于花椰菜病毒科（Caulimoviridae）杆状DNA病毒属（Badnavirus），其中7种为香蕉线条病毒（banana streak virus，BSV）。利用免疫捕捉PCR（immunocaptured PCR，IC-PCR）对宏基因组测序结果进行验证，仅检测到5种BSV，分别是香蕉线条病毒OL（banana streak OL virus，BSOLV）、香蕉线条病毒GF（banana streak GF virus，BSGFV）、香蕉线条病毒MY（banana streak MY virus，BSMYV）、香蕉线条病毒IM（banana streak IM virus，BSIMV）和香蕉线条病毒VN（banana streak VN virus，BSVNV）。利用IC-PCR扩增的这些病毒核苷酸序列与GenBank中相应病毒的一致性高于93%。系统发育进化树分析结果表明，这些病毒属于Clade I，是游离病毒。利用IC-PCR对41个广东香蕉样品进行检测，检测到BSOLV、BSGFV、BSMYV、BSIMV和BSVNV，其中BSOLV为优势BSV。

关键词: 香蕉, 杆状DNA病毒属, 香蕉线条病毒, 宏基因组测序

Abstract:

Macrogenomic sequencing was used to analyze virus infecting bananas in South China，and 16 different viruses were found，most of them belonging to the genus Badnavirus of the family Caulimoviridae. Among these，seven were banana streak virus（BSV）. To confirm the findings of macrogenomic sequencing，immunocaptured PCR（IC-PCR）was used. The results indicated that five BSVs were identified，namely banana streak OL virus（BSOLV），banana streak GF virus（BSGFV），banana streak MY virus（BSMYV），banana streak IM virus（BSIMV），and banana streak VN virus（BSVNV）. The nucleotide sequences of these viruses amplified by IC-PCR showed greater than 93% identity with the corresponding viral sequences in GenBank. Phylogenetic tree analysis revealed that these BSVs belonged to Clade I and were episomal. Additionally，BSOLV，BSGFV，BSMYV，BSIMV，and BSVNV were detected in 41 banana samples from Guangdong by IC-PCR，and BSOLV was identified as the dominant BSV.

Key words: banana, Badnavirus, banana streak virus, metagenomic sequencing

陈华宙, 刘润沛, 卢咏思, 饶雪琴. 利用宏基因组测序分析华南地区香蕉线条病毒多样性[J]. 园艺学报, 2025, 52(1): 66-72.

CHEN Huazhou, LIU Runpei, LU Yongsi, RAO Xueqin. Analysis of the Diversity of Banana Streak Virus Infecting Bananas in South China Using Metagenomic Sequencing[J]. Acta Horticulturae Sinica, 2025, 52(1): 66-72.

图/表 4

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引物名称 Primer	引物序列（5′-3′） Primer sequence	PCR产物/bp PCR product	来源 Source
BSOLV	F：TTGGTGTTTAACTATAAGAGGCTGAA；R：TTATTGCATCCACATTTGAAAAC	1 336	Delanoy et al.，2003
BSGFV	F：GCCTTCATGACCTGGCGAAT；R：ATGTACGGGTGTATTGCCGC	480	本研究This study
BSIMV	F：TCTTAGACACAGGAGCTGCCA；R：ATTCCTCCTTCCATTGCACG	256	本研究This study
BSMYV	F：TAAAAGCACAGCTCAGAACAAACC；R：CTCCGTGATTTCTTCGTGGTC	588	本研究This study
BSVNV	F：GATGGACAACTGCTTCCGAG；R：CTAAGACCATAGTTGCCTCT	490	本研究This study
BSUAV	F：GAGTATGTGCCTATGCAAGT；R：CATAGCCTTCATCTTCTGGT	680	本研究This study
BSAcYNV	F：GGTACAGAAATTGACCCCCT；R：CAGCCAGGACTGGAAGGTTG	720	本研究This study
Badv-RT	F：ATGCCNTTYGGNNTNAARAAYGCNCC；R：CCAYTTRCANACNSCNCCCCANCC	579	Rao et al.，2023

引物名称 Primer	引物序列（5′-3′） Primer sequence	PCR产物/bp PCR product	来源 Source
BSOLV	F：TTGGTGTTTAACTATAAGAGGCTGAA；R：TTATTGCATCCACATTTGAAAAC	1 336	Delanoy et al.，2003
BSGFV	F：GCCTTCATGACCTGGCGAAT；R：ATGTACGGGTGTATTGCCGC	480	本研究This study
BSIMV	F：TCTTAGACACAGGAGCTGCCA；R：ATTCCTCCTTCCATTGCACG	256	本研究This study
BSMYV	F：TAAAAGCACAGCTCAGAACAAACC；R：CTCCGTGATTTCTTCGTGGTC	588	本研究This study
BSVNV	F：GATGGACAACTGCTTCCGAG；R：CTAAGACCATAGTTGCCTCT	490	本研究This study
BSUAV	F：GAGTATGTGCCTATGCAAGT；R：CATAGCCTTCATCTTCTGGT	680	本研究This study
BSAcYNV	F：GGTACAGAAATTGACCCCCT；R：CAGCCAGGACTGGAAGGTTG	720	本研究This study
Badv-RT	F：ATGCCNTTYGGNNTNAARAAYGCNCC；R：CCAYTTRCANACNSCNCCCCANCC	579	Rao et al.，2023

采集地 Collecting site	香蕉品种 Cultivar	数量 Number	症状 Symptom	BSV检测 BSV detection
东莞Dongguan	皇帝蕉Huangdijiao	2	无症状Symptomless	—
	红蕉Hongjiao	2	无症状Symptomless	—
	贵妃蕉Guifeijiao	2	无症状Symptomless	BSMVY
广州Guangzhou	广粉1号Guangfen 1	1	无症状Symptomless	BSOLV
	粉杂1号Fenza 1	2	花叶Mosaic	—
	不详Unknown	6	花叶Mosaic	BSOLV，BSVNV
佛山Foshan	粉杂1号Fenza 1	2	无症状Symptomless	BSIMV，BSOLV
惠州Huizhou	不详Unknown	3	花叶Mosaic	—
罗定Luoding	不详Unknown	5	花叶Mosaic	BSMVY
云浮Yunfou	不详Unknown	4	花叶Mosaic	BSGFV，BSOLV
湛江Zhanjiang	不详Unknown	3	花叶Mosaic	—
茂名Maoming	不详Unknown	4	花叶Mosaic	BSMVY，BSOLV
	不详Unknown	2	无症状Symptomless	—
	南天黄Nantianhuang	1	花叶Mosaic	—
开平Kaiping	巴西蕉Brazilian banana	2	无症状Symptomless	—

采集地 Collecting site	香蕉品种 Cultivar	数量 Number	症状 Symptom	BSV检测 BSV detection
东莞Dongguan	皇帝蕉Huangdijiao	2	无症状Symptomless	—
	红蕉Hongjiao	2	无症状Symptomless	—
	贵妃蕉Guifeijiao	2	无症状Symptomless	BSMVY
广州Guangzhou	广粉1号Guangfen 1	1	无症状Symptomless	BSOLV
	粉杂1号Fenza 1	2	花叶Mosaic	—
	不详Unknown	6	花叶Mosaic	BSOLV，BSVNV
佛山Foshan	粉杂1号Fenza 1	2	无症状Symptomless	BSIMV，BSOLV
惠州Huizhou	不详Unknown	3	花叶Mosaic	—
罗定Luoding	不详Unknown	5	花叶Mosaic	BSMVY
云浮Yunfou	不详Unknown	4	花叶Mosaic	BSGFV，BSOLV
湛江Zhanjiang	不详Unknown	3	花叶Mosaic	—
茂名Maoming	不详Unknown	4	花叶Mosaic	BSMVY，BSOLV
	不详Unknown	2	无症状Symptomless	—
	南天黄Nantianhuang	1	花叶Mosaic	—
开平Kaiping	巴西蕉Brazilian banana	2	无症状Symptomless	—