https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2023, Vol. 50 ›› Issue (5): 1025-1036.doi: 10.16420/j.issn.0513-353x.2022-0177

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

月季茉莉酸羧基甲基转移酶基因RhJMT对花瓣衰老的调控

刘嘉琦1, 公菲菲1, 张颢2, 景维坤1,2, 瞿素萍2, 马男1, 高俊平1, 孙小明1,*()   

  1. 1中国农业大学园艺学院观赏园艺系,花卉发育与品质调控北京市重点实验室,北京 100193
    2云南省农业科学院花卉研究所,国家观赏园艺工程技术研究中心,昆明 650205
  • 收稿日期:2023-02-17 修回日期:2023-04-27 出版日期:2023-05-25 发布日期:2023-05-31
  • 通讯作者: *(E-mail:xmsun@cau.edu.cn
  • 基金资助:
    云南省科技厅科技计划项目(202102AE090001);国家自然科学基金青年基金项目(31902059);云南省科技厅专家工作站项目(202105AF150036)

Jasmonic Acid Carboxy Methyltransferase Gene RhJMT Regulates Petal Senescence in Rosa hybrida

LIU Jiaqi1, GONG Feifei1, ZHANG Hao2, JING Weikun1,2, QU Suping2, MA Nan1, GAO Junping1, SUN Xiaoming1,*()   

  1. 1Beijing Key Laboratory of Development and Quality Control of Ornamental Crops,Department of Ornamental Horticulture,College of Horticulture,China Agricultural University,Beijing 100193,China
    2National Engineering Research Center of Ornamental Horticulture,Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China
  • Received:2023-02-17 Revised:2023-04-27 Published:2023-05-25 Online:2023-05-31

摘要:

为了解茉莉酸甲酯(methyl jasmonate,MeJA)在花瓣衰老中的作用机制,以月季‘Samantha’为材料,在花瓣中克隆了MeJA生物合成关键酶茉莉酸羧基甲基转移酶(jasmonic acid carboxyl methyltrans-ferase,JMT)基因RhJMT。同源蛋白序列比对和系统进化树分析表明,RhJMT具有保守的Methylyransf_7超家族结构域,属于SABATH蛋白家族。实时荧光定量PCR结果表明,RhJMT的表达量在花瓣进入衰老期后显著升高。通过病毒诱导的基因沉默(virus induced gene silencing,VIGS)技术对RhJMT进行功能验证,结果表明沉默RhJMT显著延缓花瓣衰老,外源施加MeJA能够抵消沉默RhJMT对花瓣衰老的延缓作用。以上结果说明RhJMT能够促进花瓣衰老,可能是通过控制MeJA的含量来发挥作用。

关键词: 月季, 花瓣衰老, 茉莉酸甲酯(MeJA), 茉莉酸甲基转移酶(JMT), VIGS

Abstract:

In order to understand the mechanism of MeJA in petal senescence,the rose variety ‘Samantha’was used as the test material,and the jasmonic acid carboxyl methyltransferase(JMT)gene RhJMT,a key enzyme in MeJA biosynthesis,was cloned in the petals. Homologous protein sequence alignment and phylogenetic tree analysis showed that RhJMT has a conserved Methylyransf_7 superfamily domain and belongs to the SABATH protein family. The results of real-time quantitative PCR showed that the expression of RhJMT increased significantly after petal senescence. The function of RhJMT was verified by virus-induced gene silencing technology. The results showed that silencing RhJMT significantly delayed petal senescence,and that exogenous application of MeJA could counteract the delaying effect of silencing the RhJMT gene. These results indicate that RhJMT can promote petal senescence and may act by controlling the concentration of MeJA.

Key words: Rosa hybrida, petal senescence, methyl jasmonate(MeJA), jasmonate methyltransferase (JMT), VIGS

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