园艺学报 ›› 2020, Vol. 47 ›› Issue (6): 1021-1032.doi: 0.16420/j.issn.0513-353x.2019-0658

• 研究论文 •    下一篇


李泳潭,张 军*,黄亚丽,范建敏,张益文,左力辉   

  1. 河北农业大学林学院,河北省林木种质资源与森林保护重点实验室,河北保定 071000
  • 出版日期:2020-06-25 发布日期:2020-06-25
  • 基金资助:

Analysis of Chloroplast Genome of Pyrus betulaefolia

LI Yongtan,ZHANG Jun*,HUANG Yali,FAN Jianmin,ZHANG Yiwen,and ZUO Lihui   

  1. Hebei Key Laboratory for Tree Genetic Resources and Forest Protection,Forestry College,Hebei Agricultural University,Baoding,Hebei 071000,China
  • Online:2020-06-25 Published:2020-06-25

摘要: 利用高通量测序平台对杜梨(Pyrus betulaefolia)进行测序,并对其叶绿体全基因组序列的结构特征进行分析。结果表明,杜梨叶绿体基因组与大多数高等植物一样,具有典型的环状双链四分体结构。其叶绿体基因组大小为160 058 bp,共检测到61 个SSR 位点(58 个单核苷酸重复和3 个二核苷酸重复)和56 个RNA 编辑位点,这些RNA 编辑位点均引起了氨基酸的变化。与砂梨(Pyrus pyrifolia)、川梨(Pyrus pashia)和桃叶梨(Pyrus spinosa)的叶绿体基因组比较,其基因种类和数量都比较保守,表明了梨属植物进化的缓慢性。在4 个梨属植物中共检测到351 个SNP 和217 个InDel。

关键词: 杜梨, 叶绿体基因组, CP-SSR

Abstract: In this study,the chloroplast(cp)genome sequencing of Pyrus betulaeolia was done by high-throughput sequencing,and the genome sequence structure was analyzed. The results showed that like most of higher plants,cp genome had the typical circular double-chain tetrad structure. The P. betulaefolia cp genome was 160 058 bp in size,containing 61 SSR loci(58 single nucleotide repeats and 3 dinucleotide repeats)and 56 RNA editing sites,all of which caused amino acid changes. The comparison with P. pyrifolia,P. pashia,and P. spinosa showed that the genome types and numbers were relatively conservative,indicating that the Pyrus evolved slowly. A total of 351 single nucleotide polymorphisms and 217 InDels were detected in four Pyrus species.

Key words: Pyrus betulaefolia, chloroplast genome, CP-SSR