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园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2261-2266.doi: 10.16420/j.issn.0513-353x.2015-0316

• 研究报告 • 上一篇    下一篇

广东辣椒上首次检测到西瓜银斑驳病毒

汤亚飞1,何自福1,2,*   

  1. 1 广东省农业科学院植物保护研究所,广州 510640;2 广东省植物保护新技术重点实验室,广州 510640
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    国家公益性行业(农业)科研专项(201303028);广东省科技计划项目(2013B020309003)

First Report of Watermelon silver mottle virus Infecting Pepper in#br# Guangdong Province

TANG Ya-fei1 and HE Zi-fu1,2,*   

  1. 1Plant Protection Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;
    2Guangdong Provincial Key Laboratory of High Technology for Plant Protection,Guangzhou 510640,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

2014 年在广东辣椒产区发现疑似番茄斑萎病毒属(Tospovirus)病毒侵染引起的辣椒褪绿坏
死环斑症状。Dot-ELISA 检测显示,该病毒分离物与番茄斑萎病毒(Tomato spotted wilt virus,TSWV)单
克隆抗体不产生血清学反应,表明该病毒不属于TSWV。利用Tospovirus 属病毒通用引物gL3637/gL4435C
进行RT-PCR 检测,可以从所有辣椒病样总RNA 中扩增出预期大小约800 bp 的目的片段。基因克隆与序
列分析表明,该片段序列与西瓜银斑驳病毒(Watermelon silver mottle virus,WSMoV)中国广州分离物的
同源性最高,为97.5%。系统进化分析也显示,该病毒分离物与WSMoV 各分离物亲缘关系最近,并聚类
在一个分支。因此,侵染广东辣椒的病毒分离物为WSMoV。

关键词: 辣椒, 西瓜银斑驳病毒, 分子鉴定

Abstract:

Pepper plants showing chlorotic and necrotic rings symptoms on the leaves reminiscent of
infection by tospoviruses were found in pepper-production fields of Guangdong in 2014. The infected
samples were detected by Dot-ELISA and RT-PCR,respectively. Negative results were obtained in the
Dot-ELISA with monoclonal antibody of Tomato spotted wilt virus(TSWV). This result indicated that the
virus isolate didn’t belong to TSWV. However,an expected approximately 800 bp-fragment were obtained
from all infected pepper samples by RT-PCR using the universal primer pair gL3637/gL4435C for
tospoviruses. The results of gene cloning and sequence analysis showed that the fragment shared the
highest identity at 97.5% with corresponding parts of the Guangzhou isolate of Watermelon silver mottle
virus(WSMoV). Phylogenic analysis showed that the virus isolate infecting pepper in Guangdong is most
closely related to the reported isolates of WSMoV,and that they clustered in a monophyletic group.
Therefore,the virus isolate infecting pepper in Guangdong is an isolate of WSMoV.

Key words: pepper, Watermelon silver mottle virus, molecular identification

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