ISTR在部分柿属植物种质鉴定和亲缘关系分析中的应用

园艺学报 ›› 2009, Vol. 36 ›› Issue (4): 481-486.

ISTR在部分柿属植物种质鉴定和亲缘关系分析中的应用

杜晓云^1,2;张梦思¹;罗正荣¹;张青林^1*

(¹华中农业大学园艺植物生物学教育部重点实验室, 武汉430070; ²山西运城学院生命科学系, 山西运城044000)

收稿日期:2008-12-30 修回日期:2009-03-24 出版日期:2009-04-25 发布日期:2009-04-25
通讯作者: 张青林

Identif ication and Genetic Relationships of Diospyros kaki Thunb. and RelatedSpecies Using ISTR Analysis

DU Xiao-yun^1,2,ZHANG Meng-si¹,LUO Zheng-rong¹, and ZHANG Qing-lin^1*

(¹Key Laboratory of Horticultural Plant Biology Aff iliated to M inistry of Education, Huazhong Agricultural University, Wuhan 430070, China; ²Departm ent of Life and Science, Shanxi Yuncheng University, Yuncheng, Shanxi, 044000, China)

Received:2008-12-30 Revised:2009-03-24 Online:2009-04-25 Published:2009-04-25
Contact: ZHANG Qing-lin

摘要/Abstract

摘要： 利用反向序列标签重复技术( inverse sequence-tagged repeat, ISTR) , 对柿属7个种, 包括柿(Diospyros kaki Thunb. ) 、君迁子(D. lotus L. ) 、浙江柿(D. glaucifolia Metc. ) 、油柿(D. oleifera Cheng. ) 、金枣柿(D. sp. ) 、老鸦柿(D. rhombifolia Hemsl. ) 和美洲柿(D. virginiana L. ) 共32个基因型进行了种质鉴定和亲缘关系研究。结果表明: ISTR可区分供试柿属植物中的30份; ISTR能够很好地适用于柿属植物亲缘关系分析; 供试的中国与日本原产柿种质分别聚类; 新发现的中国甜柿变异类型与日本甜柿的亲缘关系较远且存在较丰富的遗传变异, 可能是潜在的育种资源。ISTR标记可在柿属植物种质资源鉴定和亲缘关系分析中更广泛地应用。

关键词: 柿属, 亲缘关系, 反向序列标签重复技术, 逆转座子分子标记

Abstract: Inverse sequence-tagged repeat (ISTR) molecularmarkerwas emp loyed to investigate genetic relationship s of 32 genotypes rep resentative of 7 species including Diospyros kaki Thunb. , D. lotus L. , D.glaucifoliaMetc. , D. oleifera Cheng. , Jinzaoshi (D. sp. ) , D. rhom bifolia Hemsl. and D. virginiana L.ISTR showed good discriminatory power between genotypes, with excep tion of one bud mutation and its original variety. The clustering results derived from ISTR molecular data was in good agreementwith the p revious studies or known genetic relationship s of some genotypes, indicating that ISTR could be app lied for the genus of Diospy ros. Both unweighted pair-group method with arithmetic averages (UPGMA) and p rincipal coordinated analysis ( PCOA) results separated Japanese persimmon into different group s, indicating their different genetic background. The new found several Chinese native PCNA varieties showed abundant genetic diversity based on ISTR analysis, aswell aswith Japanese native PCNA types, suggesting that theymay be potential and p recious breedingmaterials. In summary, ISTR would be a potential efficient tool for further germp lasm differentiation, genetic diversity assessment and phylogenetic analysis in Diospyros.

Key words: Diospyros L., phylogenetic relationship, inverse sequence-tagged repeat, retrotransposon molecular marker

中图分类号:

S 665.2

杜晓云;张梦思;罗正荣;张青林. ISTR在部分柿属植物种质鉴定和亲缘关系分析中的应用[J]. 园艺学报, 2009, 36(4): 481-486.

DU Xiao-yun;ZHANG Meng-si;LUO Zheng-rong;and ZHANG Qing-lin. Identif ication and Genetic Relationships of Diospyros kaki Thunb. and RelatedSpecies Using ISTR Analysis[J]. ACTA HORTICULTURAE SINICA, 2009, 36(4): 481-486.

[1]	张萌, 单玉莹, 杨业波, 翟飞飞, 王兆山, 巨关升, 孙振元, 李振坚. 中国石斛属植物遗传资源的AFLP分析[J]. 园艺学报, 2022, 49(6): 1339-1350.
[2]	吴致君, 李伟, 王兴华, 张成, 蒋勋, 黎盛, 罗理勇, 孙康, 曾亮. 基于SLAF-seq的茶组子房室数不稳定居群种质亲缘关系分析[J]. 园艺学报, 2022, 49(11): 2455-2470.
[3]	王鑫, 李明阳, 田琳, 刘冬云. 利用ISSR标记及rDNA-ITS序列分析河北省野生铁线莲的亲缘关系[J]. 园艺学报, 2021, 48(9): 1755-1767.
[4]	方茜，张园园，杨钰婷，黄苗苗，符巧丽，周慧莎，陈文荣，宗宇，郭卫东. 越橘叶片转录组SSR发掘及其多态性研究[J]. 园艺学报, 2018, 45(7): 1359-1370.
[5]	吴志娟，方茜，李永强，陈文荣，宗宇，郭卫东. 越橘反转录转座子插入多态性分子标记开发及品种鉴别[J]. 园艺学报, 2018, 45(4): 753-763.
[6]	王丽媛，孙鹏，张嘉嘉，傅建敏*，刁松锋，韩卫娟，索玉静，张悦. 柿野生雄性资源调查及其遗传多样性研究[J]. 园艺学报, 2018, 45(2): 261-278.
[7]	井赵斌1,3,*，徐明1,3，雷玉山1,2,3. 猕猴桃SRAP-PCR体系的建立及品种资源亲缘关系研究[J]. 园艺学报, 2016, 43(2): 337-346.
[8]	吴岐奎, 邢世岩, 王萱, 孙立民. 核用银杏品种遗传关系的AFLP分析[J]. 园艺学报, 2015, 42(5): 961-968.
[9]	岳晓燕，黄新忠，宗宇，滕元文. 福建省梨地方品种的遗传多样性研究[J]. 园艺学报, 2015, 42(1): 119-130.
[10]	董海燕1,*，季孔庶2，侯伯鑫3，赵宏波1. 基于ISSR标记的红花檵木品种亲缘关系分析[J]. 园艺学报, 2014, 41(2): 365-374.
[11]	梁晋军, 梁玉琴, 傅建敏. 基于叶绿体DNA PCR-RFLP分析柿及其近缘种亲缘关系[J]. 园艺学报, 2014, 41(12): 2474-2480.
[12]	刘有春, 陶承光, 刘威生, 刘宁, 刘硕, 章秋平, 郁香荷, 张玉萍, 徐铭, 张同喜. 花粉形态在核果类果树遗传起源和系统关系研究中的应用[J]. 园艺学报, 2013, 40(9): 1701-1709.
[13]	管志勇, 王江民, 陈发棣, 房伟民, 陈素梅, 陈春全, 俞吉钰. 基于 DUS 测试性状的切花菊品种亲缘关系研究[J]. 园艺学报, 2013, 40(7): 1399-1406.
[14]	吴娅妮, 康俊根, 王文科, 孟淑春. 菠菜种质遗传多样性和亲缘关系的AFLP 分析[J]. 园艺学报, 2013, 40(5): 913-.
[15]	张楠, 栾非时, 高鹏. 4 份野生瓜类材料抗病性及形态特征和SSR 亲缘关系研究[J]. 园艺学报, 2012, 39(5): 905-922.