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园艺学报 ›› 2008, Vol. 35 ›› Issue (7): 1081-1084.

• 研究简报 • 上一篇    下一篇

蓝猪耳45S rDNA的荧光原位杂交作图及其核型分析

陆 续;罗锐敏;高 峰*   

  1. (华南师范大学生命科学学院,广东省植物发育生物工程重点实验室,广州 510631)
  • 收稿日期:2008-04-03 修回日期:2008-06-12 出版日期:2008-07-25 发布日期:2008-07-25
  • 通讯作者: 高 峰

45S rDNA-FISH Mapping and Karyotype Analysis of Torenia fournieri L.

LU Xu, LUO Rui-min, GAO Feng*
  

  1. (Guangdong Key Laboratory of Biotechnology for Plant Development,School of Life Sciences, South China Normal University, Guangzhou 510631, China)
  • Received:2008-04-03 Revised:2008-06-12 Online:2008-07-25 Published:2008-07-25
  • Contact: GAO Feng

摘要:

以番茄45S rDNA为探针,结合核型分析和荧光原位杂交(FISH)技术,对蓝猪耳染色体的核型以及45S rDNA在染色体上的定位进行了研究。结果表明,蓝猪耳染色体的核型为1A型,核型不对称系数为57.46,染色体相对长度变异范围为4.455% ~ 6.990%,最长染色体与最短染色体的比值为1.57,染色体相对长度组成为 2L + 4M2 + 12M1,核型公式为2n=2x=18=14m + 4sm (2SAT)。在蓝猪耳体细胞有丝分裂中期染色体和间期核中,均发现45S rDNA有2个杂交信号,且信号在有丝分裂中期位于第5对染色体短臂的近着丝粒处。基于上述结果绘制了蓝猪耳染色体的核型图。

关键词: 蓝猪耳, 荧光原位杂交, 45S rDNA, 核型

Abstract:

Karyotype analysis and 45S rDNA- florescence in situ hybridization (FISH) mapping of Torenia fournieri L. were performed by using Lycopersicon esculentum 45S rDNA as a probe. The T. fournieri karyotype was classified into the type 1A in the Stebbins' category. The Asymmetry Index was 57.46. Variation in chromosome relative length ranged from 4.455% to 6.990%. The ratio of the longest over the shortest length of chromosomes (L/S) was 1.57. Chromosome constitution of relative length was 2L + 4M2 +12M1. The karyotype formula can be represented as 2n = 2x = 18 = 14m + 4sm (2SAT). Two hybridized signals were found on the chromosome 5p near the centromere at the metaphase and in the interphase nucleus of T. fournieri. An idiogram was obtained based on these findings.

Key words: Torenia fournieri L., florescence in situ hybridization, 45S rDNA, karyotype

中图分类号: