甜瓜抗蔓枯病种质资源的筛选及RAPD分析

园艺学报 ›› 2007, Vol. 34 ›› Issue (5): 1201-1206.

甜瓜抗蔓枯病种质资源的筛选及RAPD分析

周晓慧¹; Joseph N. Wolukau¹;李英¹;张永兵^1,2;吴明珠²;陈劲枫^1*

(¹作物遗传与种质创新国家重点实验室，南京农业大学园艺学院，南京 210095；²新疆农业科学院哈密瓜研究中心，乌鲁木齐 830000)

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-10-25 发布日期:2007-10-25

Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm

ZHOU Xiao-hui¹; Joseph N. Wolukau¹; LI Ying¹; ZHANG Yong-bin^1,2;WU Ming-zhu²;CHEN Jin-feng^1*

(¹State Key Laboratory of Plant Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China;²Hamigua Research Center, Xinjiang Academy of Agricultural Sciences, Urumqi830000, China)

Received:1900-01-01 Revised:1900-01-01 Online:2007-10-25 Published:2007-10-25

摘要/Abstract

摘要： 采用甜瓜蔓枯病病菌孢子悬浮液对208份甜瓜种质资源进行苗期人工接种鉴定，发现86份材料表现抗病，122份材料表现感病。用RAPD标记对抗性表现最优的29份材料进行分析，14个引物共扩增出116条多态性条带，平均每个引物为8.29条。Shannon多样性指数和Nei^,s基因多样性指数分别为0.4331和0.2855，表明这些抗源材料遗传差异较大。UPGMA聚类分析将这29份抗性种质明显划为普通型和野生型两大类，种质间还表现出一定的地域特性。

关键词: 甜瓜, 蔓枯病, 抗性, RAPD, 遗传多样性

Abstract: Two hundred and eight accessions of melon germplasm were screened and evaluated for resistance to gummy stem blight (GSB) by artificial inoculation at the seedling stage. The results showed that eighty six among them were resistant and one hundred and twenty two were susceptible. Fourteen RAPD primers were used to analyze the genetic diversity of the twenty nine accessions with the most GSB resistance. In total one hundred and sixteen DNA polymorphic bands were scored, with an average of 8.29 bands for each primer. Shannons information index (I) and Neis gene diversity index were 0.4331 and 0.2855, respectively, indicating rather large genetic distances among those germplasm. Cluster analysis by UPGMA divided these twenty nine accessions into two groups, and the accessions with the same geographical origin were clustered together.

Key words: Melon, Gummy stem blight, Resistance, RAPD, Genetic diversity

周晓慧;Joseph N. Wolukau;李英;张永兵;吴明珠;陈劲枫. 甜瓜抗蔓枯病种质资源的筛选及RAPD分析[J]. 园艺学报, 2007, 34(5): 1201-1206.

ZHOU Xiao-hui;Joseph N. Wolukau;LI Ying;ZHANG Yong-bin;WU Ming-zhu;CHEN Jin-feng. Screening and RAPD Analysis of Gummy Stem Blight Resistance in Melon Germplasm[J]. ACTA HORTICULTURAE SINICA, 2007, 34(5): 1201-1206.

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