马铃薯晚疫病水平抗性的QTL标记评价

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 391-396.

马铃薯晚疫病水平抗性的QTL标记评价

柳　俊¹;吴承金²;胡　诚¹;宋波涛¹;田振东¹;谢从华^1*

(¹华中农业大学园艺植物生物学教育部重点实验室, 国家蔬菜改良中心华中分中心, 武汉430070; ²湖北恩施州农业科学院马铃薯研究所, 湖北恩施445000)

收稿日期:2006-11-28 修回日期:2007-02-09 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 谢从华

Evalua tion of QTL Markers Associated with Horizontal Resistance to LateBlight of Potato

L IU Jun¹, WU Cheng-jin²,HU Cheng¹,SONG Bo-tao¹,TIAN Zhen-dong¹,and XIE Cong-hua^1*

[¹Key Laboratory of Horticultural Plant Biology, Ministry of Education, National Center forVegetable Im provem ent (Central China) , Huazhong Agricultural University, Wuhan 430070, China; ²Potato Research Institute, Enshi Academy of Agricultural Sciences, Enshi, Hubei 445000, China]

Received:2006-11-28 Revised:2007-02-09 Online:2007-04-25 Published:2007-04-25
Contact: XIE Cong-hua

摘要/Abstract

摘要： 以11个马铃薯晚疫病水平抗性杂交组合后代的636个基因型为材料, 对4个与晚疫病( Phytophthora infestans) 水平抗性相关位于不同染色体的QTL位点标记GP179、GP76、prp1和GP125进行了评价。结果显示: 4个QTL位点标记共有6个特征带, 在不同遗传背景的组合中带型分布存在差异; 田间抗病性与4个标记的总带数呈极显著直线相关; 主要成分分析表明, p rp1570位点标记解释了供试材料35%的抗性表型变异, 为主效QTL。结合抗性表型分析, 组合群体的抗性与QTL位点数有关, 而个体的抗病水平与主效QTL有关。

关键词: 马铃薯, 晚疫病, 水平抗性, QTL标记

Abstract: To improve the selection efficiency of potato horizontal resistance to late blight caused by Phytophthora infestans, molecularmarkers (GP179, GP76, p rp1 and GP125) of 4 quantitative trait loci (QTL)linked to potato late blight resistance and located on different chromosomeswere evaluated with 636 pedigrees
derived from 11 potato crosses with parental materials possessing horizontal resistance to late blight. The results showed that there were 6 bands produced by all the 4 markers and band distribution varied with crosses
that have different genetic background. The resistance level of the pedigrees identified in the field was significantly related to the total number of the bands yielded by the 4 markerswith a linear function. The dominant component analysis revealed that locus prp1₅₇₀ exp lained 35% of the phenotypic variance of the resistance and its linked QTL was hence classified as a dominant locus of late blight resistance. Combining the phenotypic analysis together, the experimental results have reinforced the evidence that late blight resistance is related to the number ofQTL swhen a whole potato population ( i. e. all pedigrees of a cross) is taken into account and the dominant locus determined the resistance level of individuals within this population.

Key words: Potato, Phytophthora infestans, Horizontal resistance, QTL marker

中图分类号:

S 532

柳　俊;吴承金;胡　诚;宋波涛;田振东;谢从华. 马铃薯晚疫病水平抗性的QTL标记评价[J]. 园艺学报, 2007, 34(2): 391-396.

L IU Jun;WU Cheng-jin;HU Cheng;SONG Bo-tao;TIAN Zhen-dong;and XIE Cong-hua. Evalua tion of QTL Markers Associated with Horizontal Resistance to LateBlight of Potato[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 391-396.

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