园艺学报 ›› 2005, Vol. 32 ›› Issue (02): 273-277.

• 研究论文 • 上一篇    下一篇



  1. (1 南京林业大学风景园林学院, 南京210037; 2 深圳市城市绿化管理处, 深圳518035; 3 深圳市仙湖植物园, 深圳518004)
  • 收稿日期:2004-07-01 修回日期:2004-10-22 出版日期:2005-04-25 发布日期:2005-04-25

RAPD Analysis of Germplasm Resources on Canna

Huang Guotao1;Ouyang Dimei2;Xiang Qibai1;Zhu Weihua2;Zhang Shouzhou3   

  1. (1College of Landscape, Nanjing Forestry University, Nanjing 210037, China; 2 Shenzhen Urban Garden Administration, Shenzhen 518035, China; 3 Shenzhen Fairy Lake Botanical Garden, Shenzhen 518004, China)
  • Received:2004-07-01 Revised:2004-10-22 Online:2005-04-25 Published:2005-04-25

摘要: 利用RAPD技术对美人蕉属4个种和52个品种的亲缘关系进行研究, 从125个随机引物中筛选出27个多态性较高的引物, 扩增出223条DNA带, 其中140条为多态带, 占62.78% , 平均每个引物扩增的DNA带数为8.26条。3个种和7个品种具有特异的位点, 可作为种质鉴定的依据。根据RAPD扩增结果建立美人蕉UPGMA聚类图, 在0.11处将56份种质划分为4类。分子聚类结果与形态分类结果基本一致, 4个种分属4个类群, 52个品种分为大花美人蕉和兰花美人蕉。对美人蕉品种分类和品种演化进行了初步探讨。

关键词: 美人蕉属, RAPD, 分类, 亲缘关系

Abstract: Taxonomy of four Canna species and 52 cultivarswere analyzed using random amplified polymorphic DNA. 27 p rimers were screened from 125 arbitrary 102 mer primers, and a total of 223 DNA bands were amplified, 140 of which (62.78% ) were polymorphic. The average numbers of DNA bands amplified
by each primer were 8.26. There were specific amplified bands in three species and seven cultivars, and these bands can be used in Canna germplasm identification. The dendrogram constructed by UPGMA showed that the 56 individualswere classified into four groups. Four specieswere grouped in dependently and 52 cultivars were classified into Canna ×generalis Bailey and C. ×orchioides Bailey. This conclusion consisted with the traditional Canna taxonomy. Phylogenetic relationship of Canna germplasm was also discussed.

Key words: Canna Linn., RAPD, Taxonomy, Phylogenetic relationship