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园艺学报 ›› 2026, Vol. 53 ›› Issue (9): 2609-2620.doi: 10.16420/j.issn.0513-353x.2025-0631

• 遗传育种·种质资源·分子生物学 • 上一篇    下一篇

茄子全基因组SNP变异特征及SNP-Panel开发与应用

王本启, 黄树苹, 陈霞, 谈杰, 张洪源, 王俊良, 陈蓉, 张敏*()   

  1. 武汉市农业科学院蔬菜研究所, 武汉 430065
  • 收稿日期:2025-11-13 修回日期:2026-06-02 出版日期:2026-09-25 发布日期:2026-09-20
  • 通讯作者:
  • 基金资助:
    湖北省技术创新计划重大科技项目(2025BBA001); 湖北省自然基金面上项目(2024AFB1009); 国家重点研发计划项目(2024YFD23007)

Genome-Wide SNP Variation Characteristics and Development and Application of SNP-Panel in Eggplant

WANG Benqi, HUANG Shuping, CHEN Xia, TAN Jie, ZHANG Hongyuan, WANG Junliang, CHEN Rong, ZHANG Min*()   

  1. Vegetable Research Institute, Wuhan Academy of Agricultural Sciences, Wuhan 430065, China
  • Received:2025-11-13 Revised:2026-06-02 Published:2026-09-25 Online:2026-09-20
  • Contact:

摘要:

为解析茄子基因组变异特征、开发实用分子标记并服务于品种鉴定与分子育种,以122份茄子高世代自交系为材料,采用Target-seq靶向测序技术,对茄子全基因组SNP变异特征进行分析。共鉴定出1 476个变异位点(1 433个SNP和43个InDel),其中转换型SNP占71.34%,转换/颠换比为2.50,不同染色体间SNP密度差异显著。基于此开发了含1 100个位点的SNP-Panel,该Panel在基因组上分布均匀,可用于遗传多样性分析、区分品种遗传分化、构建指纹图谱、辅助品种鉴定与保护、高效种子纯度鉴定。

关键词: 茄子, 全基因组, SNP标记, SNP-Panel, 指纹图谱, 品种鉴定, 靶向测序

Abstract:

To clarify the genomic variation characteristics of eggplant,develop practical molecular markers,and facilitate cultivar identification and molecular breeding,this study used 122 advanced-generation eggplant inbred lines as materials. Target-seq targeted sequencing technology was applied to analyze the genome-wide SNP variation characteristics of eggplant and develop SNP markers for application exploration. A total of 1 476 variant loci were identified,including 1 433 SNPs and 43 InDels. Transition-type SNP accounted for 71.34% with a transition/transversion ratio of 2.50,and the SNP density varied significantly among different chromosomes. On this basis,a SNP-Panel containing 1 100 loci was developed,which was evenly distributed across the genome. This panel can be used for genetic diversity analysis and genetic differentiation discrimination of varieties,construct DNA fingerprints to assist variety identification and protection,and efficiently conduct seed purity identification.

Key words: eggplant, whole-genome, SNP marker, SNP-Panel, fingerprint map, cultivar identification, targeted sequencing

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