文心兰OnMYB106对拟南芥育性的影响

doi:10.16420/j.issn.0513-353x.2024-0224

园艺学报 ›› 2026, Vol. 53 ›› Issue (1): 149-158.doi: 10.16420/j.issn.0513-353x.2024-0224

• 遗传育种·种质资源·分子生物学 • 上一篇下一篇

文心兰OnMYB106对拟南芥育性的影响

付帅¹^,², 许丽萍², 王周平², 叶炜³, 徐涵¹^,⁴, 叶开温¹^,⁵, 张姣⁶, 林玉玲¹^,^*, 赖钟雄¹^,^*()

¹ 福建农林大学园艺植物生物工程研究所，福州 350002
² 陕西省园艺技术工作站，西安 710000
³ 三明市农业科学研究院，福建三明 365000
⁴ 法国图卢兹综合科学研究所，法国图卢兹 31000
⁵ 台湾大学植物科学研究所，中国台北 10617
⁶ 咸阳市农业科学研究院，陕西咸阳 712000

收稿日期:2025-05-16 修回日期:2025-12-01 出版日期:2026-01-25 发布日期:2026-01-26
通讯作者:
^*E-mail：laizx01@163.com
^*E-mail：laizx01@163.com
基金资助:
福建省重大农业科技专项(2015NZ-0002-1); 福建省高原学科建设经费项目(102/71201801101)

Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

FU Shuai¹^,², XU Liping², WANG ZhouPing², YE Wei³, XU Han¹^,⁴, YE Kaiwen¹^,⁵, ZHANG Jiao⁶, LIN Yuling¹^,^*, LAI Zhongxiong¹^,^*()

¹ Institute of Horticultural Plant Bioengineering， Fujian Agriculture and Forestry University， Fuzhou 350002， China
² Shaanxi Horticultural Technology Workstation， Xi’an 710000， China
³ Sanming Agricultural Sciences Research Institute， Sanming， Fujian 365000， China
⁴ Institute of Comprehensive Science in Toulouse， France， Toulouse 31000， France
⁵ Institute of Plant Science， Taiwan University， Taipei 10617， China
⁶ Xianyang Academy of Agricultural Sciences， Xianyang， Shaanxi 712000， China

Received:2025-05-16 Revised:2025-12-01 Published:2026-01-25 Online:2026-01-26

摘要/Abstract

摘要：

分析文心兰‘柠檬绿’（Oncidium‘Honey Angel’）OnMYB106和拟南芥AtMYB106编码的蛋白序列、保守结构域和理化性质等，推测二者功能相似。以AtMYB106的拟南芥突变体（myb106）为研究对象，并对其遗传转化OnMYB106（35S:OnMYB106-myb106），对比分析野生型、突变体（myb106）和遗传转化OnMYB106的恢复株（35S:OnMYB106-myb106）三者的表型、抽薹天数、莲座叶片数和结荚情况等。结果表明：野生型、myb106和35S: OnMYB106-myb106的抽薹时间分别为32、28和31 d；抽薹时莲座叶片数分别为12、8和12片；抽薹时莲座叶冠直径分别为4.1、2.8和3.5 cm；单株植物结荚数分别为64、15和42个。其中野生型果荚内含种子数约为19粒，果荚长度约为1.07 cm，myb106显著低于野生型，分别约为7粒和0.75 cm，35S:OnMYB106-myb106为18粒和0.81 cm，三者整体结荚能力由高到低排序依次为野生型 > 35S: OnMYB106-myb106 > myb106。

关键词: 文心兰, 拟南芥, OnMYB106, 育性

Abstract:

By analyzing the protein sequences，conserved domains，and physicochemical properties of OnMYB106 from Oncidium（Honey Angel）and AtMYB106 from Arabidopsis thaliana，it is speculated that the functions of the two are similar. The mutant of the AtMYB106（myb106）was used as the research object，and it was genetically transformed with OnMYB106（35S:OnMYB106-myb106）. The phenotypes days to bolting、number of rosette leaves and pod-setting of the wild type，myb106 and 35S: OnMYB106-myb106 were comparatively analyzed. The results showed that the bolting times for the wild-type，myb106 and 35S:OnMYB106-myb106 were 32，28 and 31 d，respectively；the number of rosette leaves at bolting was 12，8 and 12 pcs，respectively；and the rosette leaf canopy diameters at bolting were 4.1，2.8 and 3.5 cm，respectively. The number of siliques per plant was 64，15 and 42 siliques，respectively. The wild-type siliques contained approximately 19 seeds each and were about 1.07 cm in length. These values were significantly lower in the myb106，with approximately 7 seeds and 0.75 cm length，respectively. The 35S:OnMYB106-myb106 complementation line showed values of 18 seeds and 0.81 cm. Overall，the silique production capacity of the three lines，ranked from highest to lowest，was：wild-type > 35S:OnMYB106-myb106 > myb106.

Key words: Oncidium, Arabidopsis, OnMYB106, fertility

付帅, 许丽萍, 王周平, 叶炜, 徐涵, 叶开温, 张姣, 林玉玲, 赖钟雄. 文心兰OnMYB106对拟南芥育性的影响[J]. 园艺学报, 2026, 53(1): 149-158.

FU Shuai, XU Liping, WANG ZhouPing, YE Wei, XU Han, YE Kaiwen, ZHANG Jiao, LIN Yuling, LAI Zhongxiong. Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana[J]. Acta Horticulturae Sinica, 2026, 53(1): 149-158.

图/表 9

表1 本研究中所用引物相关信息

Table 1 Primers information used in this study

引物名称 Primer name	引物序列（5′-3′） Primer sequence	退火温度/℃ Annealing temperature
AtMYB106-LP	ATACTCACTCTCATTCCCCACG	60
AtMYB106-RP	GCTCCTCAACAACATTGCTTC	60
BP	GCGTGGACCGCTTGCTGCAACT	60
Q-GUS-F	CGACTGGGCAGATGAACAT	60
Q-GUS-R	ATACTCCACATCACCACGCT	60
RT-OnMYB106-F	GAAGAGCTGTAGGTTGAGGTGGACGA	60
RT-OnMYB106-R	CGGGTCAATCCCCATCTTTGC	58
RT-AtMYB106-F	ACCAAGGAAACGGAGACCAAC	60
RT-AtMYB106-R	ATCTGCCTAATCCAGTCGTGTT	60
Actin-F	GCAACATTGTTCTTAGCGGAGGCT	60
Actin-R	TCTTCATGCTGCTTGGTGCAAGTG	60

图1 文心兰和拟南芥蛋白保守结构域与核定位信号预测（A）和Motif分析（B） On：文心兰；At：拟南芥

Fig. 1 Prediction of conserved protein domains，nuclear localization signal structure（A） and Motif analysis（B）of Oncidium and Arabidopsis thaliana On：Oncidium；At：Arabidopsis thaliana

图2 AtMYB106基因结构和插入位点的分析（A）及鉴定结果（B、C）

Fig. 2 Analysis of AtMYB106 gene structure and insertion site（A）and identification results（B，C）

图3 myb106突变体过表达OnMYB106植株的GUS检测 1 ~ 8：转基因阳性植株的GUS检测条带

Fig. 3 GUS detection of myb106 mutant overexpressing OnMYB106 plants 1-8：GUS detection bands of transgenic positive plants

图4 AtMYB106在myb106中（A）和OnMYB106在35S:OnMYB106-myb106中（B）的表达量分析

Fig. 4 Analysis of the expression levels of AtMYB106 in myb106（A）and OnMYB106 in 35S:OnMYB106-myb106（B）

图5 野生型、myb106和35S-OnMYB106-myb106突变体的表型分析

Fig. 5 Phenotypic analysis of wild type，myb106 and 35S-OnMYB106-myb106 mutant in Arabidopsis thaliana

图6 野生型（WT）、myb106、35S:OnMYB106-myb106植株抽薹时的性状分析

Fig. 6 Phenotypic analysis of wild-type（WT），myb106 and 35S:OnMYB106-myb106 Arabidopsis at bolting

表2 野生型、myb106和35S:OnMYB106-myb106的果荚数据分析

Table 2 Analysis of silique of wild type，myb106 and 35S:OnMYB106-myb106

基因型 Genotype	果荚数 Number of per silique	种子数 Number of seeds	果荚长/cm Silique length
野生型Wild type	64.08 ± 7.85	19.91 ± 6.03	1.12 ± 0.09
myb106	12.00 ± 3.00**	7.00 ± 2.00**	0.75 ± 0.19**
35S: OnMYB106-myb106	42.25 ± 7.90*	18.16 ± 1.82	0.81 ± 0.90*

图7 拟南芥开放期花药和花粉粒的亚历山大染色箭头表示败育花粉粒

Fig. 7 Alexander staining of anthers and pollen grains in Arabidopsis during anther opening Arrows indicate aborted pollen grains

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文心兰OnMYB106对拟南芥育性的影响

Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

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文心兰OnMYB106对拟南芥育性的影响

Effect of Oncidium OnMYB106 on Fertility of Arabidopsis thaliana

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