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园艺学报 ›› 2022, Vol. 49 ›› Issue (1): 148-156.doi: 10.16420/j.issn.0513-353x.2020-1018

• 研究报告 • 上一篇    下一篇

虎奶菇菌丝体和菌核基因组甲基化分析

务俊月, 孙雪言, 杨振华, 罗璐, 莫翠园, 马爱民()   

  1. 华中农业大学食品科学技术学院,武汉 430070
  • 收稿日期:2021-05-08 修回日期:2021-11-08 出版日期:2022-01-25 发布日期:2022-01-24
  • 通讯作者: 马爱民 E-mail:aiminma@mail.hzau.edu.cn
  • 基金资助:
    国家自然科学基金项目(31772375)

Methylation Analysis of Mycelium and Sclerotium Genome in Pleurotus tuber-regium

WU Junyue, SUN Xueyan, YANG Zhenhua, LUO Lu, MO Cuiyuan, MA Aimin()   

  1. College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China
  • Received:2021-05-08 Revised:2021-11-08 Online:2022-01-25 Published:2022-01-24
  • Contact: MA Aimin E-mail:aiminma@mail.hzau.edu.cn

摘要:

以虎奶菇[Pleurotus tuber-regium(Fr.)Singer]菌丝体和菌核为材料,利用MSAP技术分析其甲基化水平,并对甲基化差异片段进行回收测序,探究虎奶菇生长与DNA全基因组甲基化的关系。结果表明,菌丝体和菌核的DNA甲基化率分别为7.94%和9.54%,其中半甲基化率分别为4.50%和4.09%,全甲基化率分别为3.44%和5.45%。即菌核形成伴随甲基化水平的提高,全甲基化水平更能代表总体甲基化状态。对部分甲基化差异片段回收、克隆及测序,并经NCBI同源性比对,发现涉及的同源基因或蛋白主要与细胞生长、代谢、抗逆等相关,推测虎奶菇可能通过甲基化调控这些基因或蛋白的表达形成菌核来适应环境变化。

关键词: 虎奶菇, 菌丝体, 菌核, DNA甲基化, 甲基化敏感扩增多态性

Abstract:

The mycelium and sclerotium of Pleurotus tuber-regium(Fr.)Singer were used as materials,the methylation levels were analyzed by MSAP technology,and the differentially methylated fragments were recovered and sequenced to explore the relationship between the growth of P. tuber-regium and DNA whole genome methylation.The results showed that the DNA methylation rates of mycelium and sclerotium were 7.94% and 9.54%,respectively;the hemi-methylation rates were 4.50% and 4.09%,and the fully methylation rates were 3.44% and 5.45%,respectively.That is,the formation of sclerotium was accompanied by an increase in the methylation level,the full methylation level was more representative of the overall methylation status.Partially methylated differential fragments were recovered,cloned and sequenced.The NCBI homology comparison showed that the homologous genes or proteins are mainly related to metabolism,cell growth,and stress resistance.It is speculated that P. tuber-regium might regulate the expression of these genes or proteins through methylation to form sclerotium to adapt to environmental changes.

Key words: Pleurotus tuber-regium, mycelium, sclerotium, DNA methylation, methylation-sensitive amplified polymorphism(MSAP)

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