https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2016, Vol. 43 ›› Issue (5): 966-974.doi: 10.16420/j.issn.0513-353x.2016-0046

• 研究报告 • 上一篇    下一篇

‘冬枣’天然二、四混倍体的发现与评价

刘 颖1,冯春芳1,于洪长2,王玖瑞1,*,刘孟军3,*   

  1. (1河北农业大学林学院,河北保定 071001;2山东沾化冬枣研究所,山东沾化 256803;3河北农业大学中国枣研究中心,河北保定 071001)
  • 出版日期:2016-05-25 发布日期:2016-05-25

Discovery and Evaluation of Natural Mixoploid(2x + 4x)Variants in Ziziphus jujuba Mill.‘Dongzao’

LIU Ying1,FENG Chun-fang1,YU Hong-chang2,WANG Jiu-rui1,*,and LIU Meng-jun3,*   

  1. (1College of Forestry,Agricultural University of Hebei,Baoding,Hebei 071001,China;2Zhanhua Institute of Dongzao,Zhanhua,Shandong 256803,China;3 Research Center of Chinese Jujube,College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071001,China)
  • Online:2016-05-25 Published:2016-05-25

摘要:

以山东省沾化县栽培的普通‘冬枣及其3个大果型变异类型‘沾冬2号、变异1号、变异2号为试材,对其染色体倍性、DNA指纹、果实和叶片形态及果实主要营养指标进行了比较分析。经流式细胞仪测定,3个大果型变异类型均为二、四混倍体(2x + 4x),其对照(母株)均为纯二倍体。经74对SSR引物检测,未发现3个大果型混倍体变异与其二倍体对照的DNA指纹存在差异,表明三者均确系来源于普通的二倍体‘冬枣’,其四倍体部分均属于同源加倍。与普通‘冬枣相比,3个变异类型均表现出叶片变宽,叶形指数变小,果实变大,果形指数变小,果实维生素C含量增加,但果实可溶性糖和可滴定酸含量变化无规律。与已通过良种审定的‘沾冬2号相比,新发现的变异1号和变异2号的单果质量更大,分别增大22.95%和15.90%,分别达到27.05 g和25.50 g。

关键词: 枣, 变异, 四倍体, 混倍体

Abstract:

Comparisons of chromosome ploidy,DNA fingerprint,fruit and leaf morphology,and fruit nutrition index were carried out among the common‘Dongzao’cultivated in Zhanhua County,Shandong,and three bigger-fruit variants,i.e.‘Zhandong 2,variant No.1 and No.2. The results showed that the three bigger-fruit variants were all mixoploids(2x + 4x)according to flow cytometry analysis,and their mother plants were pure diploids. No differences of DNA fingerprint were observed using 74 pairs of SSR primers between the three bigger-fruit variants and their diploid controls(common‘Dongzao’),indicating that they were really originated from the common diploid‘Dongzao’and their 4x parts were autotetraploids. Compared to their diploid controls(common‘Dongzao’),the three mixploid variants had bigger and broader fruits with smaller fruit shape index,wider leaves with smaller leaf shape index,and significant higher content of vitamin C in fruit. However,the soluble sugar and titrable acid contents in fruits did not 

always change in accordance with the variations of ploidy. Compared toZhandong 2having been released as a new excellent cultivarthe average fruit weight of variant No.1 and No.2 increased by 22.95% and 15.90% and reached 27.05 g and 25.50 grepectively.

Key words: Chinese jujube, variant, tetraploid, mixoploid

中图分类号: