Abstract: In order to study the metabolic mechanism related to flower development in Eriobotrya japonica，GC–MS was used as the detection technique. The metabolites in five different developmental processes were detected at physiological differentiation stage，morphological differentiation stage，inflorescence emergence and expansion stage，single flower development stage and blossoming stage. Multivariate statistical analysis was carried out for all the samples，and then the metabolites were statistically classified and the most relevant metabolic pathways were screened out. In this research，the metabolic phenotypes of different flower developmental stages were summarized and analyzed. The soluble sugar，soluble proteins and starch content as well as C/N ratio variation during the five stages were also detected. A total of 430 metabolites were obtained. Amino acid and carbohydrate metabolism were the two most significant metabolic pathways related to flower development，and the metabolism was more vigorous in single flower development stage than the other stages. The soluble sugar，soluble proteins，starch content and C/N ratio were consistent with the phenotypic results of the metabolome. C/N ratio is one of the important factors determining thedevelopment process of flowers.

Key words: Eriobotrya japonica, flower, metabolic, amino acids, carbohydrate