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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1437-1445.doi: 10.16420/j.issn.0513-353x.2020-0668

• Research Papers •     Next Articles

Cloning and Expression Analysis of the Cytokinin Response Regulator VlRR5 in Kyoho Grapevine

BIAN Lu, GUO Dalong, YU Keke, WEI Tonglu, PEI Maosong, LIU Hainan, YU Yihe()   

  1. College of Horticulture and Plant Protection,Henan University of Science and Technology,Luoyang,Henan 471023,China
  • Received:2021-02-23 Revised:2021-06-07 Online:2021-08-25 Published:2021-09-06
  • Contact: YU Yihe E-mail:yuyihe@haust.edu.cn

Abstract:

There are three fruit-dropping peaks in grape growth and development. Grape fruit-dropping phenomenon is more serious in Kyoho grapevine. In this study,a cytokinin response regulator VlRR5 was cloned from Kyoho grapevine,which has a phosphoric acid receptor domain(REC)and a MYB-like DNA binding domain. Sequence analysis revealed that cDNA of VlRR5 was 2 597 bp in length,encoding 693 amino acids,and located on chromosome 4 of grape. Subcellular localization assay showed that VlRR5 was localized in the nucleus. The yeast transformed into pGBKT7-VlRR5 could grow normally and turn blue on SD/-Trp/-Ade/-His/+X-α-Gal deficient medium,which proved that VlRR5 was a transcription factor with transcriptional activation activity. By quantitative real-time PCR analysis,VlRR5 was characterized by tissue specific expression and showed high expression level in leaf and stem,which was increased and then decreased in the development stage of young fruit. The expression level increased after treatment with exogenous cytokinin,and decreased after treatment with the cytokinin synthesis inhibitor lovastatin. These results indicated that VlRR5 can respond to cytokinin and plays an important role in participating in cytokinin to improve the fruiting rate of grape.

Key words: grapevine, cytokinin, CPPU, cytokinin response regulators, VlRR5

CLC Number: