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园艺学报 ›› 2006, Vol. 33 ›› Issue (3): 642-644.

• 研究简报 • 上一篇    下一篇

植物生长调节剂对羽衣甘蓝小孢子胚发生的影响

姜凤英1, 2;冯 辉1*   

  1. (1沈阳农业大学园艺学院, 辽宁沈阳110161; 2 辽宁省农业科学院花卉研究所, 辽宁沈阳110161)
  • 收稿日期:2005-07-25 修回日期:2005-10-20 出版日期:2006-06-25 发布日期:2006-06-25

Effect of the Auxin and Ctytokin on the Frequency of Embryogenesis in Kale

Jiang Fengying1, 2;Feng Hui1*   

  1. (1 College of Horticulture, Shenyang Agricultural University, Shenyang, Liaoning 110161, China; 2 Floriculture Institute of Liaoning Academy of Agricultural Science, Shenyang, Liaoning 110161, China)
  • Received:2005-07-25 Revised:2005-10-20 Online:2006-06-25 Published:2006-06-25

摘要: 以‘圆叶红心’和‘皱叶红心’2个羽衣甘蓝F1杂交种为试材, 进行游离小孢子培养, 研究了6-BA、NAA和2, 4-D对其胚状体诱导的影响, 结果表明: 6-BA对诱导胚状体起促进作用, 其适宜浓度为0.2 mg·L - 1 ; NAA抑制小孢子胚状体发生, 但能促进其发育, 提高正常胚比例; 2, 4-D有较强的抑制作用, 只有少量细胞团的形成; 6-BA、NAA综合作用能显著提高胚状体诱导率, 其浓度配比以1∶1或2∶1为佳。

关键词: 羽衣甘蓝, 小孢子培养, 胚状体, 生长素, 细胞分裂素

Abstract: The effects of 6-BA, NAA and 2, 4-D on embryoid induction during adopting isolated microspore culture of kale (Brassica oleracea L. var. acephala DC. ) were studied using two hybrids‘Yuanye Hongxin’and ‘Zhouye Hongxin’. The results showed that 6-BA could promote embryoid induction and the suitable content was 0.2 mg·L - 1. NAA could not p romote embryoid formation, but increased the proportion of normal embryoid. 2, 4-D has significant inhibition to embryoid formation and only produce a little agglomerate cells. In addition, the media with 62BA and NAA dramatically increased the frequency of microspore-derived embryoids and the suitable content ration was 1∶1 or 2∶1.

Key words: Kale, Microspore culture, Embryoid, Auxin, Ctytokin