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园艺学报 ›› 2020, Vol. 47 ›› Issue (8): 1463-1474.doi: 10.16420/j.issn.0513-353x.2019-0965

• 研究论文 • 上一篇    下一篇

枣AP2/ERF转录因子鉴定及其响应枣疯病植原体表达分析

李继东*,倪 静*,叶 霞,陈 鹏,谭 彬,张梦洋,郑先波,张 玉,冯建灿**   

  1. 河南农业大学,河南省果树瓜类生物学重点实验室,郑州 450002
  • 出版日期:2020-08-25 发布日期:2020-08-25
  • 基金资助:
    河南省重大科技专项(151100110900);河南省现代农业产业体系大宗水果产业技术创新团队项目(S2014-11-G02)

Genomic Identification of Jujube AP2/ERF Transcription Factors and Their Expression Pattern During Jujube Witches’ Broom Pathogenesis Process

LI Jidong*,NI Jing*,YE Xia,CHEN Peng,TAN Bin,ZHANG Mengyang,ZHENG Xianbo,ZHANG Yu,and FENG Jiancan**   

  1. Henan Agricultural University,Henan Key Laboratory of Fruit and Cucurbit Biology,Zhengzhou 450002,China
  • Online:2020-08-25 Published:2020-08-25

摘要: 利用生物信息学方法,从植物转录因子数据库和NCBI数据库中分别得到175和164个候选的枣AP2/ERF转录因子序列,使用DNAMAN软件进行序列比对、去除重复序列,采用SMART软件预测蛋白结构域发现,枣基因组中包含有145个AP2/ERF基因,其中ERF、AP2、RAV亚家族分别含有116个、23个、5个,另有1个独立基因。预测枣AP2/ERF转录因子氨基酸数量在111 ~ 692之间,分子量在12 446.87 ~ 76 154.10之间,pI在4.31 ~ 10.11之间。鉴定出的145个AP2/ERF转录因子,105个分别定位到12条染色体上,40个未能定位。在此基础上,利用嫁接病芽方法将枣疯病植原体转至健康枣植株,通过转录组测序和qRT-PCR手段,分析了AP2/ERF转录因子对枣植原体侵染的响应,在植原体侵染枣的6个不同时期,枣AP2/ERF表达数量和表达量均不相同,共有48个差异表达基因,其中ZjAP2*9、ZjERF49和ZjERF91是响应枣疯病植原体最为重要的AP2/ERF转录因子。

关键词: 枣, 枣疯病, 植原体, AP2/ERF转录因子

Abstract: In this study,a total of 175 and 164 jujube AP2 / ERF transcription factors were obtained from the plant transcription factor database and the NCBI database by bioinformatics methods,respectively. Duplicated sequences were deleted after sequences aligning by DNAMAN. ERF domain were confirmed by SMART software. Finally,145 AP2/ERF transcription factors were identified,of which 116,23,and 5 members were divided into ERF,AP2,RAV subfamily,respectively,including another independent gene. The jujube AP2/ERF transcription factors were featured with amino acid numbers among 111–692,molecular weight among 12 446.87–76 154.10 D,and isoelectric point among 4.31–10.11. 105 jujube AP2/ERF genes were mapped to the 12 jujube chromosomes and the others were not. Jujube witches’ broom(JWB) diseased buds were grafted to healthy 1-year-old seedlings and 4-year-old trees to perform an infection process. The response of AP2/ERF transcription factors to JWB phytoplasma was analyzed according to the transcriptome datas and qRT-PCR. Results showed that the expressing level and number of AP2/ERF were different in 6 sampling stages. The expressing level of 48 AP2/ERF were significantly different in 6 periods. Among them,ZjAP2*9,ZjERF49 and ZjERF91 were the most important AP2/ERF transcription factors in response to the phytoplasma.

Key words: jujube, jujube witches’ broom, phytoplasma, AP2/ERF transcription factors

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