http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
http://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2017, Vol. 44 ›› Issue (11): 2163-2170.doi: 10.16420/j.issn.0513-353x.2017-0280

• 研究报告 • 上一篇    下一篇

苹果MdNAC143 的克隆及其在苹果愈伤组织的抗盐功能鉴定

张全艳,于建强,王佳慧,胡大刚*,郝玉金*   

  1. 山东农业大学园艺科学与工程学院,作物生物学国家重点实验室,农业部黄淮地区园艺作物生物学与种质创制重
    点实验室,山东泰安 271018
  • 出版日期:2017-11-25 发布日期:2017-11-25

Molecular Cloning and Functional Characterization of MdNAC143 Reveals#br# Its Involvement in Salt Tolerance in Apple Callus

ZHANG Quanyan,YU Jianqiang,WANG Jiahui,HU Dagang*,and HAO Yujin*   

  1. State Key Laboratory of Crop Biology,MOA Key Laboratory of Horticultural Crop Biology(Huanghuai Region) and
    Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai'an,
    Shandong 271018,China
  • Online:2017-11-25 Published:2017-11-25

摘要:

从‘嘎拉’苹果中克隆了一个NAC 转录因子基因MdNAC143(序列号:MDP0000334047),
其开放阅读框为924 bp,编码308 个氨基酸,预测蛋白质的分子量为35.59 kD,等电点为6.32。结构域
分析表明,MdNAC143 蛋白N 端含有保守的NAC 结构域。酵母双杂交结果表明MdNAC143 的全长及C
端具有转录激活活性。荧光定量PCR 分析表明,MdNAC143 在苹果的各个组织均有表达,其中在叶片和
花中表达相对较高;MdNAC143 的表达明显受盐胁迫的诱导。将MdNAC143 遗传转化‘王林’苹果愈伤
组织,进行抗盐表型鉴定后发现,MdNAC143 在愈伤组织中过量表达后能明显提高对盐胁迫的抗性。

关键词: 苹果, MdNAC143, 基因表达, 转录激活, 盐胁迫

Abstract:

A NAC transcription factor(TF)(Genbank accession number:MDP0000334047)was
cloned from Malus × domestica‘Royal Gala’. Sequence analysis showed that the ORF of the MdNAC143
was 924 bp,which encoded 308 amino acids. It was predicted that the molecular mass of this protein was
35.59 kD and the pI was 6.32. Analysis of functional domain showed that the MdNAC143 protein included
about the conserved NAC domain. The results of yeast two-hybrid showed that the full length and
C-terminal of MdNAC143 had transcriptional activation activity. qRT-PCR analysis showed that the
MdNAC143 gene was generally expressed in all tissues of apple and the expression was significantly
higher in leaves and flowers. Meanwhile,the expression of MdNAC143 was induced by salt stress.
Finally,salt-tolerance assay indicated that overexpression of MdNAC143 remarkably increased the
tolerance of transgenic apple callus to high salinity.

Key words: apple, MdNAC143, gene expression, transcriptional activation, salt stress