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园艺学报 ›› 2014, Vol. 41 ›› Issue (10): 2085-2093.

• 观赏植物 • 上一篇    下一篇

外源ABA对叶子花开花及内源ABA合成关键酶的影响

赵家昱1,潘远智1,李永红2,*   

  1. 1四川农业大学风景园林学院,成都 611130;2深圳职业技术学院应用化学与生物技术学院,广东深圳 518055
  • 收稿日期:2014-05-08 出版日期:2014-10-25 发布日期:2014-10-25
  • 基金资助:
    深圳市科技项目(2112K3070004)

Effects of Abscisic Acid on Flowering and Some Key Enzymes of Bougainvillea glabra

ZHAO Jia-yu1,PAN Yuan-zhi1,and LI Yong-hong2,*   

  1. 1College of Landscape Architecture,Sichuan Agricultural University,Chengdu 611130,China;2School of Applied Chemistry and Biotechnology,Shenzhen Polytechnic,Shenzhen,Guangdong 518055,China
  • Received:2014-05-08 Online:2014-10-25 Published:2014-10-25

摘要: 以叶子花‘大红宝巾’(Bougainvillea glabra‘Mrs Butt’)为试材,研究外源脱落酸(ABA)对其开花及其生物合成关键酶含量与活性变化的影响。结果表明:外源ABA对叶子花的成花有促进作用;去甲二氢愈创木酸(NDGA)浓度大于10 μmol ? L-1时,ABA的合成明显被抑制,低浓度NDGA与高浓度ABA共同作用反而对内源ABA含量的升高具有增效作用;内源ABA的变化趋势与9–顺式–环氧类胡萝卜素双氧合酶(NCED)含量的变化趋势相一致,表明NCED为ABA生物合成途径中的限速酶;外源ABA使玉米黄质环氧化酶(ZEP)、NCED、ABA醛氧化酶(AAO)含量及活性均明显上升,表明ABA能自我催化调节其合成,且单一酶基因或单一酶促反应并不能合理解释ABA水平的变化,推测ABA信号的作用机制是一个由多酶有机统筹系统控制的结果。

关键词: 叶子花, 脱落酸, 玉米黄质环氧化酶(ZEP), 9–顺式–环氧类胡萝卜素双氧合酶(NCED), 脱落酸醛氧化酶(AAO)

Abstract: Bougainvillea glabra was chosen as subjects and treated by different concentration of nordihydroguaiaretic acid(NDGA)and abscisic acid(ABA). Their flowering time was observed and the concentration of zeaxanthin epoxidase(ZEP),9-cis-epoxycarotenoid dioxygenase(NCED),ABA aldehyde oxidase(AAO)and ABA in the leaf were detected. Experiment result showed that ABA had a significant promotion of the plant’s florescence,while NDGA(> 10 μmol ? L-1)reduced the concentration of ABA. External ABA stimulated the enzymatic activity and concentration of ZEP,NCED and ZEP. It demonstrates that ABA can mediate its synthesis by autocatalystic reaction and single enzyme or single enzymatic reaction couldn’t reasonably explain the change of ABA level. It is concluded that ABA signal mechanism is a result of coordination and system control by a multienzyme system.

Key words: Bougainvillea glabra, ABA, ZEP, NCED, AAO

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