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园艺学报 ›› 2014, Vol. 41 ›› Issue (8): 1620-1630.

• 蔬菜 • 上一篇    下一篇

甘蓝自交系背景选择标记的建立

刘基生,苗雯雯,王冬梅,庄 木*,方智远,刘玉梅,杨丽梅,张扬勇,李占省   

  1. (中国农业科学院蔬菜花卉研究所,北京 100081)
  • 收稿日期:2014-04-19 出版日期:2014-08-25 发布日期:2014-08-25
  • 基金资助:
    国家重点基础研究发展计划(‘973’)项目(2012CB1139);国家‘863’计划课题(2012AA100101,2012AA100202);国家现代农业产业技术体系建设专项资金项目(CARS-25);中央级公益性科研院所基本科研业务费专项资金项目(1610032011011,014JB02-004);农业部园艺作物生物学与种质创制重点实验室项目

Establishment of Markers for Genetic Background Selection of Inbred Lines in Cabbage

LIU Ji-sheng,MIAO Wen-wen,WANG Dong-mei,ZHUANG Mu*,FANG Zhi-yuan,LIU Yu-mei,YANG Li-mei,ZHANG Yang-yong,and LI Zhan-sheng   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
  • Received:2014-04-19 Online:2014-08-25 Published:2014-08-25

摘要: 为了快速、准确识别不同甘蓝自交系的遗传背景,提高回交育种效率,利用36对多态性EST-SSR引物对8个甘蓝变种的101份自交系进行扩增,应用NTSYSpc2.11a进行遗传背景分析表明,不同甘蓝变种种内平均遗传相似系数都大于0.55,变种间遗传相似系数为0.45 ~ 0.59,101份自交系在遗传相似系数0.62处基本可聚为8个类群。在此基础上,选出23份具有代表性的自交系材料,增加筛选获得多态性稳定的50对EST-SSR引物,初步建立了一套包含86对引物的甘蓝背景选择标记,并应用于甘蓝自交亲和性的回交选择。

关键词: 甘蓝, 自交系, 背景选择, EST-SSR

Abstract: For the purpose of the rapid and accurate identification of genetic background of cabbage inbred lines and the improvement of backcross breeding efficiency,we performed PCR amplification of 36 primer pairs in 101 inbred lines from eight varieties in Brassica oleracea and analyzed their genetic background using software NTSYSpc2.11a. Results showed that all the average coefficients of intra- variety for the eight varieties are bigger than 0.55,and that of inter-variety vary from 0.45 to 0.59. The 101 B. oleracea accessions can be divided into eight groups with the coefficient of 0.62. Based on these results,23 out of 101 inbred lines were selected and 50 EST-SSR markers were added. At last,a set of 86 markers for genetic background selection was preliminarily obtained and used successfully for the backcross selection of cabbage self-compatibility.

Key words: cabbage, inbred line, background selection, EST-SSR

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