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园艺学报 ›› 2014, Vol. 41 ›› Issue (6): 1115-1124.

• 蔬菜 • 上一篇    下一篇

白菜种子萌发的热抑制现象及其与细胞壁降解酶的关系

宋顺华1,宋松泉2,*,吴 萍1,孟淑春1,邢宝田1   

  1. (1北京市农林科学院蔬菜研究中心,农业部华北地区园艺作物生物学与种质创制重点实验室,农业部都市农业(北方)重点实验室,北京 100097;2中国科学院植物研究所,北京 100093)
  • 收稿日期:2014-02-18 出版日期:2014-06-25 发布日期:2014-06-25

Thermoinhibition of Brassica rapa ssp. chinensis Seed Germination in Relation to Degrading Enzymes of Cell Walls

SONG Shun-hua1,SONG Song-quan2,*,WU Ping1,MENG Shu-chun1,and XING Bao-tian1   

  1. (1Beijing Vegetable Research Center,Beijing Academy of Agriculture and Forestry Sciences,Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(North China)and Urban Agriculture(North),Ministry of Agriculture,Beijing 100097,China;2Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China)
  • Received:2014-02-18 Online:2014-06-25 Published:2014-06-25

摘要: 为了探讨白菜种子萌发热抑制的机制,以新收获的白菜种子为材料,研究了种子萌发对不同温度的响应,后熟、冷层积和植物激素处理对种子萌发的作用,以及种子萌发和不同处理与细胞壁降解酶之间的关系。结果表明,当萌发温度 ≥ 20 ℃时,种子的萌发率显著降低,热抑制的种子不表现次生休眠。后熟、层积和GA3处理能有效地降低种子萌发的热抑制;相反,ABA处理则增强种子萌发的热抑制。种子萌发过程中内切–β–甘露聚糖酶、β–甘露糖苷酶和α–半乳糖苷酶的活性增加。与新收获的种子相比,后熟、层积和GA3处理增加上述3种酶的活性;ABA处理增加内切–β–甘露聚糖酶的活性,但不影响β–甘露糖苷酶和α–半乳糖苷酶的活性。在不同温度下吸胀48 h的种子的内切–β–甘露聚糖酶和α–半乳糖苷酶的活性变化与随后种子的萌发没有直接关系。说明新收获的白菜种子具有明显的萌发热抑制,这种特性能被后熟、层积和GA3处理以增强细胞壁降解酶活性的方式有效地解除。

关键词: 白菜, 种子, 萌发热抑制, 植物激素, 后熟, 层积, 细胞壁降解酶

Abstract: In order to study mechanism of seed germination thermoinhibition,the mature Brassica rapa ssp. chinensis seeds were used to investigate the response of seed germination to different temperatures,the effect of after-ripening,cold stratification and phytohormones to seed germination,and the relationship between seed germination and different treatments and degrading enzymes of cell walls. The results showed that seed germination was significantly decreased at ≥ 20 ℃ and in darkness,but the thermoinhibited seeds did not exhibit secondary dormancy. After-ripening,stratification and GA3 treatment effectively decreased thermoinhibition of seed germination,in contrast ABA treatment notably increased thermoinhibition. Activities of endo-β-mannanase,β-mannosidase and α-galactosidase increased during seed germination. Compared with freshly-collected seeds,treatments of after-ripening,stratification and GA3 increased the activities of above three enzymes,ABA treatment increased endo-β-mannanase activity and did not alter β-mannosidase and α-galactosidase activities. There was no direct correlation between seed germination and change in endo-β-mannanase and α-galactosidase activities of seeds imbibed at different temperatures for 48 h. The results indicated that freshly-collected B. rapa seeds had a typical germination thermoinhibition,which can be broken by after-ripening,stratification and GA3 treatments in an increasing the degrading enzyme activity of cell walls.

Key words: Brassica rapa ssp. chinensis, seed, germination thermoinhibition, phytohormone, after-ripening, stratification;degrading enzyme of cell walls

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