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园艺学报 ›› 2013, Vol. 40 ›› Issue (2): 255-264.

• 蔬菜 • 上一篇    下一篇

设施番茄长期连作土壤微生物群落结构及多样性分析

 马宁宁, 李天来   

  1. 沈阳农业大学园艺学院,辽宁省设施园艺重点实验室,沈阳 110866
  • 出版日期:2013-02-25 发布日期:2013-02-25

Effect of Long-term Continuous Cropping of Protected Tomato on Soil
Microbial Community Structure and Diversity

 MA  Ning-Ning, LI  Tian-Lai   

  1. College of Horticulture,Shenyang Agricultural University,Liaoning Province Key Laboratory of Protected Horticulture,
    Shenyang 110866,China
  • Online:2013-02-25 Published:2013-02-25

摘要: 利用PCR-DGGE 技术,研究了裸地及不同连作年限的设施番茄栽培土壤中细菌和真菌的群
落结构及多样性的变化情况。结果表明:设施条件下种植番茄明显改变了土壤土著细菌的群落结构,但
连作年限对土壤细菌多样性影响较小,细菌群落结构变化不大;土著真菌的群落结构稳定性优于土著细
菌,但土壤真菌的优势种群在不同连作年限的土样中变化较大,连作显著降低了某些真菌的数量,同时
显著增加了另一些真菌的数量,其中,连作20 年番茄的土壤真菌新出现的优势种群最多,且与非优势种
群的真菌数量差异较大,这种番茄连作后改变土壤真菌种群平衡的现象可能是导致番茄产生连作障碍的
重要原因之一。通过电泳条带的回收、测序,可知所取土样中的细菌优势种群属于Clostridium butyricum
Uncultured bacteriumBacillusArthrobacter,真菌优势种群属于Saccobolus dilutellusUncultured fungus
Lasiobolus ciliatusSpooneromyces laeticolorLasiobolidium orbiculoides

关键词: 番茄, 长期连作, 土壤微生物, PCR-DGGE, 群落结构, 多样性

Abstract: Community structure and diversity of microorganism in bare soil and continuous cropping
protected tomato field were studied with PCR-DGGE technology. Results showed that soil indigenous
bacterial community structure was significantly influenced by planting tomato under protected field,but
soil bacterial community structure and diversity were not sensitive to continuous cropping years.
Compared with soil indigenous bacteria,indigenous fungal community structure was more steady,but the
dominant fungi were more different in the fields with different continuous cropping years,continuous
cropping significantly decreased the number of some fungi,meanwhile,the number of some other fungi
was significantly increased,most dominant fungi populations were enriched in soil with tomato continuous
cropping years of 20,and which number was quite different with the non-dominant fungi populations. The
256 园 艺 学 报 40 卷
change of soil fungi population balance that caused by tomato continuous cropping maybe one of the
important reasons for the tomato continuous cropping obstacle. Recycling and sequence analysis of
prominent electrophoretic bands showed that the dominant bacteria of soil sample belong to Clostridium
butyricum
Uncultured bacteriumBacillus and Arthrobacter,while the dominant fungus belong to
Saccobolus dilutellusUncultured fungusLasiobolus ciliatusSpooneromyces laeticolor and Lasiobolidium
orbiculoides.

Key words: tomato, long-term continuous cropping, soil microbial, PCR-DGGE, community
structure,
diversity

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