君子兰花器官离体培养的初步研究

园艺学报 ›› 2007, Vol. 34 ›› Issue (6): 1563-1568.

君子兰花器官离体培养的初步研究

邢桂梅;毕晓颖;雷家军^*

(沈阳农业大学园艺学院，沈阳 110161)

收稿日期:2007-04-19 修回日期:2007-09-01 出版日期:2007-12-25 发布日期:2007-12-25
通讯作者: 雷家军

Floral Organ in Vitro Culture of Clivia miniata Regei

XING Gui-mei, BI Xiao-ying, and LEI Jia-jun^*

(College of Horticulture, Shenyang Agricultural University, Shenyang 110161, China)

Received:2007-04-19 Revised:2007-09-01 Online:2007-12-25 Published:2007-12-25

摘要/Abstract

摘要： 以君子兰品种‘油匠’的花瓣、花丝、胚珠等花器官为外植体进行离体培养，结果表明：采用0.1%升汞消毒10 min，花器官外植体污染率较低，仅为9.9%；不同花器官外植体愈伤组织诱导与分化能力不同，花瓣的愈伤组织诱导率和分化率最高，分别达到15.6%和57.1%；花瓣外植体诱导愈伤组织和分化成苗的能力与花蕾大小、植物生长调节剂及蔗糖浓度等因素有关，最适培养基为MS+2,4-D 2.0 mg·L^-1+BA 1.0 mg·L^-1+NAA 0.5 mg·L^-1+蔗糖3.0%，适宜的花蕾长度为0.6~1.0 cm。蔗糖浓度为3%时，花器官外植体愈伤组织诱导率与分化成苗率较高。

关键词: 君子兰, 花器官, 离体培养

Abstract: The petal, filament and ovule of Clivia miniata Regei 'Youjiang' were used as explants for in vitro culture. The results showed that the explants disinfected with 0.1% HgCl₂ for 10 min were most effective with the average contamination rate of 9.9%.The rates of induction and differentiation of three kinds of floral organ explants were different. The petals had the highest induction and differentiation rates of 15.6% and 57.1%, respectively. There was direct relationship between percentage of induction and differentiation of petals and size of flower buds, concentration of hormone and sucrose. The rates of callus induction and differentiation were the highest when the length of flower bud was 0.6-1.0 cm. The optimum medium was MS+2,4-D 2.0 mg·L^-1+BA 1.0 mg·L^-1+NAA 0.5 mg·L^-1+3.0% sucrose. The rate of induction and differentiation of floral organ explants was much higher while the concentration of sucrose was 3%.

Key words: Clivia miniata Regei, Floral organ, Tissue culture

中图分类号:

S 682.1⁺3

邢桂梅;毕晓颖;雷家军. 君子兰花器官离体培养的初步研究[J]. 园艺学报, 2007, 34(6): 1563-1568.

XING Gui-mei;BI Xiao-ying;and LEI Jia-jun. Floral Organ in Vitro Culture of Clivia miniata Regei[J]. ACTA HORTICULTURAE SINICA, 2007, 34(6): 1563-1568.

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