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园艺学报 ›› 2015, Vol. 42 ›› Issue (8): 1569-1575.doi: 10.16420/j.issn.0513-353x.2014-1141

• 研究报告 • 上一篇    下一篇

安徽及周边地区枣种质资源遗传多样性研究

孙俊,孙雯雯,周军永,周张彬,陆丽娟,孙其宝   

  1. 1安徽农业大学园艺学院,合肥 230036;2安徽省农业科学院园艺研究所,合肥 230031
  • 出版日期:2015-08-25 发布日期:2015-08-25
  • 基金资助:
    安徽省自然科学基金项目(1308085MC48);国家科技部农业成果转化项目(2013GB2C300212;2010GB2C300191);科技部科技基础性工作专项(2012FY110100)

Jujube Germplasm from Anhui Province and Provinces Around Anhui Analyzed by ISSR Markers

SUN Jun, SUN Wen-Wen, ZHOU Jun-Yong, ZHOU Zhang-Bin, LU Li-Juan, SUN Qi-Bao   

  1. 1Horticulture College,Anhui Agricultural University,Hefei 230036,China;2Horticulture Research Institute,Anhui Academy of Agricultural Sciences,Hefei 230031,China
  • Online:2015-08-25 Published:2015-08-25

摘要: 利用ISSR标记对32份安徽省地方枣种质资源及山东、河南等邻近省份的42份枣资源进行了遗传多样性分析。26条引物在74份种质中共扩增出241个位点,其中多态性位点214个,多态性比率为88.8%。利用NTSYS软件计算得到DICE相似系数为0.5936 ~ 0.9353;32份安徽地方种质共扩增出219条电泳谱带,其中186条为多态性条带,多态性比率为84.93%,32份样品遗传相似系数在0.6267 ~ 0.9353之间,表明安徽地方枣种质资源遗传多样性较为丰富。采用UPGMA法对74份种质的ISSR数据进行聚类分析,分析结果显示可分为8个类群,种质间亲缘关系与地理来源关系较为密切,来自安徽的地方种质基本上都独立聚成不同的类群,表明安徽地方枣种质与周边其它省份枣种质资源亲缘关系较远;研究结果表明体细胞突变可能是推动安徽地方枣种质进化的重要因素。

关键词: 枣, 资源, 遗传多样性, 安徽

Abstract: Thirty-two jujube(Ziziphus jujuba Mill.)accessions from Anhui Province and fourty-two jujube cultivars from provinces around Anhui were analyzed by ISSR markers. A total of 241 markers were amplified from the 26 primers,of which 214 bands were polymorphic bands(the percentage of polymorphic band,PPB = 88.8%). Revealed by NTSYS software,the DICE coefficient of 74 accessions ranged from 0.5936–0.9353. Thirty-two jujube accessions from Anhui Province amplified 219 loci,of which 186 loci were polymorphic(PPB = 84.93%). Analyzed by NTSYS,the DICE coefficient among 32 accessions from Anhui Province ranged from 0.6267–0.9353,reflecting the high genetic diversity of jujube germplasms in Anhui Province. The UPGMA dendrogram demonstrated that the 74 accessions were clustered into 8 groups which were basically consistent with geographical origin. Most accessions from Anhui Province were clustered into independent groups which were far from other accessions and indicated that mutation promoted the evolution of jujube germplasm in Anhui Province.

Key words: Ziziphus jujuba, germplasm, genetic diversity, Anhui Province

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