关键词: 库尔勒香梨, MYB基因, 启动子, 激素, 调控元件

Abstract: The flowers of Korla Fragrant pear（Pyrus sinkiangensis Yu）were used as materials to make sure the relationship between Korla Fragrant pear fruit calyx shed or persistence and the expression of the kfpMYB gene，according to kfpMYB gene’s cDNA sequence and pear genomic information designed a pair of primers，a genomic DNA sequence with a length of 1 659 bp and an upstream regulatory sequence with a length of 2 440 bp were obtained by PCR，the promoter’s cis-acting elements were analyzed and predicted using PLACE and PlantCare databases. Bioinformatics analysis showed that the kfpMYB promoter sequence included transcriptional regulatory elements related to hormonal regulation，including three ABA-responsive cis-acting elements（ABRERATCAL，DPBFCOREDCDC3，and EBOXBNNAPA），three GA-responsive elements（WRKY71OS，GARE-motif，and TATC-box），and two auxin-responsiveelements（NTBBF1ARROLB and TGA-element）. Real-time PCR was used to determine the effects of different hormones and growth regulators on the transcriptional level of the kfpMYB gene. The results showed that ABA，ETH，GA，NAA and fruit control agent（PBO）affected the expression of the kfpMYB gene. In conclusion，the results indicate that transcriptional regulatory elements are involved in the expression of the kfpMYB gene.

Key words: Pyrus sinkiangensis, MYB gene, promoter, hormone, regulatory element