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园艺学报 ›› 2007, Vol. 34 ›› Issue (3): 729-734.

• 观赏植物 • 上一篇    下一篇

红掌四倍体的离体诱导及其鉴定

张志胜1*; 黎扬辉2; 姜 蕾1; 李 运1; 王朱莹1; 夏 晴2; 易懋升2
  

  1. (1 华南农业大学广东省植物分子育种重点实验室, 广州510642; 2 广州花卉研究中心, 广州510360)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-25 发布日期:2007-06-25

In Vitro Tetraploid Induction and Its Identif ication in Anthurium andraeanum

ZHANG Zhi-sheng1*; L I Yang-hui2; J IANG Lei1; LI Yun1; WANG Zhu-ying1; XIA Qing2; YI Mao-sheng2
  

  1. (1Guangdong Provincial Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642,China;2Guangzhou Flower Research Center, Guangzhou 510360,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

摘要: 以愈伤组织为诱导材料, 采用秋水仙素处理方法进行了红掌四倍体的离体诱导。结果发现,
红掌四倍体诱导率因处理方法、秋水仙素浓度和处理时间不同而异。采用液体培养的四倍体诱导率比固体
培养的高, 低浓度秋水仙素的诱导率比高浓度的高, 长时间处理的诱导率比短时间处理的高, 基因型对四
倍体诱导率影响不明显。在含0.2 g·L-1秋水仙素的液体培养基中振荡培养14 d, 四倍体诱导率最高, 为
45.5%。四倍体红掌的花药和花粉比二倍体的大, 植株粗壮, 叶片和佛焰苞大而厚, 颜色深, 叶片下表皮
上的气孔密度小, 长度长。改良压片法与气孔长度和密度鉴定法是鉴定红掌四倍体的可靠方法。

关键词: 红掌, 多倍体, 气孔鉴定, 细胞学鉴定, 愈伤组织

Abstract: Calli were employed to establish the technical system of tetraploid induction in Anthurium an
draeanum
by using colchicine treatment method. The results showed that the induction rate of tetrap loid in
vitro depended on treatmentmethod, concentration of colchicine and duration of treatment. The lower the con
centration of colchicine was, the higher the induction rate was. At the same concentration of colchicine, the
induction rate of tetrap loid using liquid culture was higher than that using solid culture, and the longer the du
ration of treatment was, the higher the induction rate was. The highest induction rate of tetrap loid, 45.5% ,
was obtained from the calli cultured in the liquid medium supp lemented with 0.2 g·L-1 colchicine for 14 d
on a rotator. The induction rate was not affected by genotype. Tetrap loid of Anthurium and raeanum was char
acterized by larger and thicker leaves and spathes with deeper color and larger size of pollen, anther and
p lant. Its length of stomatal cell on lower ep idermis leaf cellwas longer than that of diploid, while the density
of stomata was less than that of diploid. This indicated that tetrap loids of An thu rium andraeanum could be pro
duced by colchicine treatment and accurately determined by the improved squash method, the length of stoma2
tal cell and the density of stomata.

Key words: Anthurium andraeanum, Polyploid, Stomatal appraisal, Cytological appraisal, Callus