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园艺学报 ›› 2019, Vol. 46 ›› Issue (8): 1531-1542.doi: 10.16420/j.issn.0513-353x.2018-0963

• 研究论文 • 上一篇    下一篇

甜瓜syntaxin家族基因鉴定及其对白粉病菌响应分析

董玉梅,孙建磊,王崇启,肖守华,高 超*,焦自高*   

  1. 山东省农业科学院蔬菜花卉研究所,国家蔬菜改良中心山东分中心,山东省设施蔬菜生物学重点实验室,农业部黄淮地区蔬菜科学观测实验站(山东),济南250100
  • 出版日期:2019-08-25 发布日期:2019-08-25
  • 基金资助:

    国家重点研发计划项目(2018YFD0100705);国家现代农业产业技术体系建设专项资金项目(CARS-25);山东省自然科学基金项目(ZR2018LC017);山东省农业科学院农业科技创新工程项目(CXGC2018E08)

Identification and Expression Analysis of the syntaxin Family Genes Response to Powdery Mildew Infection in Melon

DONG Yumei,SUN Jianlei,WANG Chongqi,XIAO Shouhua,GAO Chao*,and JIAO Zigao*   

  1. Shandong Key Laboratory of Greenhouse Vegetable Biology,National Improvement Center for Vegetable,Shandong Branch,Vegetable Science Observation and Experiment Station in Huanghuai District of Ministry of Agriculture(Shandong),Institute of Vegetables and Flowers,Shandong Academy of Agricultural Sciences,Jinan 250100,China
  • Online:2019-08-25 Published:2019-08-25

摘要:

为了揭示突触融合蛋白(syntaxin)家族基因在甜瓜抵御白粉病过程中的功能,采用生物信息学方法从甜瓜基因组序列中鉴定到17个syntaxin家族成员,这些基因不均匀地分布在9条染色体上。系统进化分析发现甜瓜17个syntaxin家族成员属于7个亚家族(SYP-1、SYP-2、SYP-3、SYP-4、SYP-5、SYP-7、SYP-8),表明不同syntaxin家族成员之间具有不同的分子功能。基因结构分析表明甜瓜syntaxin家族基因包含1 ~ 12个内含子。组织表达分析表明有10个甜瓜syntaxin家族基因组成性表达,7个基因组织特异性表达。此外,甜瓜幼苗经白粉病菌人工接种侵染之后,9个syntaxin家族基因呈现不同程度的响应,9个syntaxin家族基因与MLO家族基因共表达,推测其在甜瓜抗白粉病过程中发挥重要功能。

关键词: 甜瓜, syntaxin, 进化分析, 表达分析, 白粉病

Abstract:

The aim of this study was to gain the information about syntaxin family genes involved in resisting to powdery mildew infection in melon. A total of 17 syntaxin family genes were identified in the genome of melon using bioinformatics approach,which were randomly and unevenly distributed on nine respective chromosomes. Phylogenetic analysis indicated that melon syntaxin proteins could be classified into seven main groups(SYP-1,SYP-2,SYP-3,SYP-4,SYP-5,SYP-7,SYP-8),suggesting that each syntaxin functions in different pathways. This gene family in melon contained 1–12 introns. Besides,10 syntaxin genes significantly expressed in almost all tissues and seven syntaxin genes expressed only in specific tissue. Furthermore, nine syntaxin genes were up or down-regulated following powdery mildew infection and nine genes were co-expressed with CmMLO family genes in melon,indicating these genes might play important roles in resisting to powdery mildew disease in melon.

Key words: melon, syntaxin, phylogenetic analysis, expression analysis, powdery mildew disease

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