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园艺学报 ›› 2019, Vol. 46 ›› Issue (10): 1936-1946.doi: 10.16420/j.issn.0513-353x.2018-0571

• 研究论文 • 上一篇    下一篇

龙眼TFL1同源基因在拟南芥和烟草中过量表达的功能研究

张艺勇,彭 媛,付志远,曾黎辉*   

  1. 福建农林大学园艺学院,福州 350002
  • 出版日期:2019-10-25 发布日期:2019-10-25
  • 基金资助:
    福建省高原学科建设经费(102/71201801101)

Functional Analysis of Longan TFL1 Homologous Genes by Over- expression in Arabidopsis thaliana and Tobacco

ZHANG Yiyong,PENG Yuan,FU Zhiyuan,and ZENG Lihui*   

  1. College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,China
  • Online:2019-10-25 Published:2019-10-25

摘要: 为了研究龙眼TFL1同源基因DlTFL1-1和DlTFL1-2的功能,构建植物表达载体pCAMBIA2300- DlTFL1-1和pCAMBIA2300-DlTFL1-2,利用根癌农杆菌GV3101介导将DlTFL1-1和DlTFL1-2分别转化拟南芥和烟草,观察基因过量表达对拟南芥和烟草开花时间和表型的影响。DlTFL1-1和DlTFL1-2蛋白与AtTFL1有高度相似的二级结构,并具有典型的PEBP保守结构域,但是在蛋白质三级结构上明显不同。在长日照和短日照条件下,DlTFL1-1和DlTFL1-2转基因拟南芥都出现了开花延迟的现象,长日照下比野生型对照分别延迟了10.0和6.8 d,短日照下分别延迟了5.3和3.0 d;转基因拟南芥花序分支明显增多。DlTFL1-1和DlTFL1-2转基因烟草也出现了开花延迟的现象。qPCR结果显示长日照下DlTFL1-1和DlTFL1-2转基因拟南芥叶片中AtFT的表达量显著下调,花序中AtLEAFY和AtAP1的表达量显著下调。试验结果表明DlTFL1-1和DlTFL1-2具有抑制花芽分化的功能,可能是龙眼中的开花抑制基因。

关键词: 龙眼, TFL1同源基因, 开花抑制基因

Abstract: In order to study the functions of longan TFL1 homologs DlTFL1-1 and DlTFL1-2,Plant expression vectors pCAMBIA2300-DlTFL1-1 and pCAMBIA2300-DlTFL1-2 were constructed and DlTFL1-1 and DlTFL1-2 were transformed into Arabidopsis and tobacco mediated by Agrobacterium tumefaciens GV3101. Effects of overexpression of DlTFL1-1 and DlTFL1-2 on flowering time and phenotypes of transgenic Arabidopsis and tobacco plants were observed. D1TFL1-1 and D1TFL1-2 proteins had a highly similar secondary structure with AtTFL1 and had a typical PEBP conserved domain,however,D1TFL1-1 and D1TFL1-1 proteins have significantly different protein tertiary structure with AtTFL1. The flowering time was delayed in both DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis under both long-day and short-day conditions. Under long-day conditions,the flowering time of DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis was delayed for 10.0 and 6.8 days,respectively. At the same time,DlTFL1 transgenic Arabidopsis plants had more branches in inflorescences. Under short-day conditions,the flowering time of DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis was delayed for 5.3 and 3.0 days respectively. In addition,the delay of flowering time was also observed in DlTFL1-1 and DlTFL1-2 transgenic tobacco plants. qPCR results showed that under long-day conditions,the expression level of AtFT in leaves decreased significantly. The expression of AtLEAFY and AtAP1 in inflorescences were also significantly down-regulated. Our results suggested that DlTFL1-1 and DlTFL1-2 play an role in inhibiting floral bud differentiation,and they may be the flowering repressors in longan.

Key words: longan, TFL1 homologous gene, flowering repressing gene

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