http://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
http://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
http://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
http://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
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http://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
http://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
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http://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
http://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2015, Vol. 42 ›› Issue (11): 2229-2236.doi: 10.16420/j.issn.0513-353x.2015-0252

• 观赏植物 • 上一篇    下一篇

牡丹隐花色素基因PsCRY2 的克隆与表达分析

任秀霞,王顺利,薛璟祺,朱富勇,刘传娇,张秀新   

  1. 中国农业科学院蔬菜花卉研究所,农业部园艺作物生物学与种质创制重点实验室,北京 100081
  • 出版日期:2015-11-25 发布日期:2015-11-25
  • 基金资助:

    中国农业科学院院所基金项目(1610092015002-01);国家‘863’计划项目(2011AA10020703);国家公益性行业(农业)
    科研专项(201203071);中国农业科学院科技创新工程项目(CAAS-ASTIP-IVFCAAS)

Molecular Cloning and Expression Analysis of Cryptochrome Gene PsCRY2#br# in Tree Peony

REN Xiu-xia,WANG Shun-li,XUE Jing-qi,ZHU Fu-yong,LIU Chuan-jiao,and ZHANG Xiu-xin   

  1. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops,Ministry of Agriculture,P. R. China,
    Institute of Vegetables and Flowers,Chinese Academy of Agricultural Science,Beijing 100081,China
  • Online:2015-11-25 Published:2015-11-25

摘要:

以‘秋发1 号’和‘洛阳红’牡丹(Paeonia suffruticosa)为试验材料,采用RT-PCR 的方
法从花芽中克隆得到1 个隐花色素基因Cryptochrome 2,其ORF 全长为1 902 bp,编码633 个氨基酸,基
因登录号为KP982893。序列比对和结构域分析表明,此蛋白包含1 个PHR 和1 个CCT 结构域,与拟南
芥中AtCRY2 最为相似,将其命名为PsCRY2。系统进化树分析表明,PsCRY2 与甜橙(Citrus sinensis)
CsCRY2 亲缘关系最近。实时荧光定量PCR 表明,PsCRY2 在牡丹的不同组织器官中均有表达。其中,成
年植株的花芽以及种子胚的表达量最高,幼苗根、茎、叶次之。在整个花芽分化不同时期,PsCRY2 的表
达呈现较高的表达水平;在花蕾发育不同时期,PsCRY2 的表达呈现先降低再升高而后有降低的趋势,大
风铃期表达量最高,推测PsCRY2 在花芽分化和花蕾发育的过程中均起到重要作用。在‘洛阳红’和‘秋
发1 号’牡丹春季花芽发育过程中,PsCRY2 的表达均呈升高的趋势,‘秋发1 号’显著高于‘洛阳红’。
不同光周期条件下,PsCRY2 表达稍有不同。与长日照条件相比,短日照条件下PsCRY2 在现蕾期和小风
铃期的表达量均下降。

关键词: 牡丹, 光周期途径, 隐花色素, PsCRY2, 实时荧光定量PCR

Abstract:

Cryptochrome 2(CRY2)was isolated from Paeonia suffruticosa by RT-PCR. The full open
reading fragment(ORF)length of PsCRY2 encoding 633 amino acids was 1 902 bp,and the GenBank No.
was KP982893. Sequence alignment and motif analysis showed that the deduced amino acids contained a
PHR domain near the amino terminus and a CCT domain near the carboxy terminus. It was designated as
PsCRY2,according to its high identity with AtCRY2 in Arabidopsis. Phylogenetic analysis showed that it
had close relationship with Citrus sinensis. Gene expression analysis revealed that the highest expression
levels of PsCRY2 were appeared in the bud and seed embryo of P. suffruticosa,followed by root,stem and
leaves. The expression of PsCRY2 in the whole process of bud differentiation was high. While the
expression of PsCRY2 was low in the early stage of bud development and quite high in the middle and latestage. The expression of PsCRY2 reached a peak in big bell-like flower-bud. The results suggested that
PsCRY2 play an important role both in the process of bud differentiation and bud development. The
expression patterns of PsCRY2 in the bud of‘Luoyanghong’and‘Qiufa 1’were similar,while the
expression level of PsCRY2 in the bud of‘Qiufa 1’was significantly higher than that in the bud of
‘Luoyanghong’. The expression patterns of PsCRY2 in the buds with different photoperiod treatments
were different. The expression of PsCRY2 decreased in bud sprouting and small bell-like flower bud under
short day photoperiod,compared with that in long day photoperiod.

Key words: tree peony, photoperiod pathway, cryptochromes, PsCRY2, qRT-PCR

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