Abstract: A 626 bp fragment of replication protein region of TYLCV（Tomato yellow leaf curl virus） was cloned using PCR with primers JC1 from Shijiazhuang samples with typical symptoms of yellow leaf curl，and sequence analysis confirmed that it was a part of TYLCV. Then the full-length of TYLCV was obtained with a pair of primers QC and sequenced. The already reported universary primers CR01/CR02 for DNA-B were used for amplification and the results showed that only DNA-A containing 2 781 bp nucleotides and it was named as TYLCV-shijiazhuang（GenBank accession No. KF612971）. Sequenceanalysis showed that TYLCV-shijiazhuang was similar to TYLCV-Israel for 99.0%. However，it was sometimes expceptional that TYLCV-Shijiazhuang isolate was most closely related to TYLCV-Beijing3（GenBank accession No. GU983859），TYLCV-SDSG-XC（GenBank accession No. KC999851），and they all belonged to the same monophyletic clusters of TYLCV-Israel isolate.

Key words: tomato, TYLCV, DNA-A, molecular identifcation, sequence analysis