Abstract:

In a culture experiment, shoot apexes were the best explant and 1/2 MS was the best basal medium. Adventitious buds and calli were induced from shoot apexes of Clematis ‘Multi-Blue’which were cultured on solid 1/2MS medium containing 0.5 mg·L-1TDZ+0.01 mg? L-1NAA+2% sucrose and 0.8 mg·L-1TDZ+0.5 mg·L^-1NAA+2%sucrose respectively. Induction rate of buds is up to 200.8% after one month and the buds were vigorous. Somatic embryos were obtained from the grey yellow lumped and granular calli through successive transfer culture. The induction rate of somatic embryos was up to 60%, and it can be developed to normal seedlings in the same medium.

Key words: Clematis, Basic Media, Adventitious Buds, Embryogenesis