Abstract: An efficient p lant regeneration p rotocol for Phyllanthus em blica L. was achieved via organo2
genesis from callus tissues formed on hypocotyl exp lants in vitro. In this paper, several factors affecting in vitro
regeneration of Phy llanthus em blica L. were examined. The op timum medium for shoot regeneration was 1 /2
MSmedium containing 011μmol·L^-1 NAA, 5μmol·L - 1 BA, 40 g·L^-1 sucrose, 1 mg·L^-1 AgNO₃. The
op timum exp lants were upper segments of hypocotyl of 282day-old seedlings. Under 14 d dark treatment,
100% of adventitious shoot regeneration frequency with 1117 shoots per exp lantwas obtained. Excised shoots
rooted when theywere transferred to 1 /2MSmedium containing 3 mg·L^-1 22 ( diethylamino) ethyl ester cit
rate (DA26) + 3 mg·L^-1 compound sodium nitrophenolate (CSN ) after 35 days and developed into
healthy p lantlets, which resulted in a rooting rate of 62.8%. The results of this studywill facilitate the app li
cation of genetic transformation methods in Phyllanthus emblica L.

Key words: Phyllanthus emblica L., Callus, Plant growth regulator, Hypocotyl, Shoot regeneration