Abstract:

The unpollinated ovules of cucumber（Cucumis sativus L.）line 65G × 228 were used to
obtain haploid plants via in vitro gynogenesis. With the unfertilized ovules，a regeneration system of
cucumber was established by analizing the effects of pretreatment temperature，induction medium，
concentration of TDZ，KT and BA on haploid induction. The results indicated that 0.02 mg · L-1 TDZ was
better for the initiation of gynogenesis. Induction medium and concentration of KT significantly affected
the frequency of embryo induction. Further，the highest embryo regeneration frequency was achieved at
IM3 medium supplemented with 0.75 mg · L-1 KT and 2.0 mg · L-1 BA. The embryos could grow root and
develop into the complete plant on MS medium. Flow cytometry analysis showed that 18.2% of the
regenerated plants were haploid.

Key words: cucumber, unfertilized ovule, haploid, flow cytometry