Abstract: The cucumber wild variety‘PI183967’（Cucumis sativus var. hardwickii）and a cultivated
cucumber Northern Chinese inbred line‘931’were used as parental materials to construct genetic
populations because of their significant difference in seed size. The inheritance of seed length and width
was studied by joint segregation analysis using positive and negative six-generation populations. A SSR
genetic map was constructed using the RILs to finish QTL mapping of seed length，width and 100-seed
weight. Results showed that（1）The inheritance of seed length and width fitted into C–0 model（Additive
dominance-epistasis polygene inheritance model）in the positive and negative cross six-generation，and
mainly with additive effect of multi-genes（2）A SSR linkage map of cucumber was constructed 149 SSR
markers. The map included 9 linkages and spanned 389.2 cM with a mean marker interval of 2.61 cM.（3）
14 QTLs related to seed length，seed width and 100-seed weight were detected 2012 and 2013 spring.
These QTLs accounted for 7.4%–28.3% of phenotypic variance were located on Chr.2，Chr.3，Chr.4，Chr.5，Chr.6 with LOD scores of 2.59–9.39. Of all the QTLs，6 QTLs explained over 10.0% of phenotypic
variance accounting for 42.9% and 3 QTLs could be detected in different years which accounted for
21.5%.

Key words: cucumber, seed size, genetic analysis, QTL mapping