Abstract: To innovate chrysanthemum germplasm by introducing elite attributes of its allied genera，artificial hybridizations have been made between Dendranthema morifolium‘Tianzhuiyulu’（2n = 54；♀）and three intergeneric hybrids（♂）. The seed set of D. morifolium ‘Tianzhuiyulu’ ×（D. crassum × Crossostephium chinense），D. morifolium‘Tianzhuiyulu’× （D. indicum × C. chinense）and D. morifolium ‘Tianzhuiyulu’×（D. nankingense × Tanacetum vulgare）was 22.5%，12.5% and 0.25% respectively. Dual-color genomic in situ hybridization was applied for these hybrids. In D. morifolium‘Tianzhuiyulu’ × （D. indicum × C. chinense）hybrid，it contained two genomes of D. morifolium‘Tianzhuiyulu’and D. indicum. In D. morifolium‘Tianzhuiyulu’× （D. nankingense × T. vulgare）hybrid，it contained two genomes of D. morifolium‘Tianzhuiyulu’and D. nankingense. While in D. morifolium‘Tianzhuiyulu’×（D. crassum × C. chinense）hybrids，they contained three genomes of D. morifolium‘Tianzhuiyulu’，D. crassum and C. chinense. The morphology of the hybrid and its parents differed significantly from one another.

Key words: Chrysanthemum, Dendranthema, allied genera, distant hybridization, dual-color genomic in situ hybridization