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ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (6): 1539-1542.

• 研究简报 • Previous Articles     Next Articles

Studies of Indirect Somatic Embryogenesis of Papaya

RAO Xue-qin and LI Hua-ping*   

  1. (College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China)
  • Received:2007-03-30 Revised:2007-09-12 Online:2007-12-25 Published:2007-12-25

Abstract: In order to establish genetic transformation system of foreign genes on small fruit type of papaya, indirect somatic embryogenesis of immature embryos (90-120 days after pollination) of Carica papaya L. 'Meizhonghong' was studied by histological section and observation with a scanning electron microscope. The results showed that the calli were originated from the epidemic cells of the radicle after 8-12 days of culture. After 26 days of culture, the whole embryonary axis turned into calli which differentiated to translucent and mucous, and dry types. The cross-section results showed the calli consisted of two distinct sectors, a loose friable tissue of outer sectors and compact aggregates of inter sectors. Yellow pre-embryonic complexes were derived from calli of the plumula after 38 days of culture. It was observed that most of morphological somatic embryos were normally developed.

Key words: Carica papaya L., Papaya, Somatic embryogenesis

CLC Number: