Studies on the Genetic Diversity of Some Chestnut (Castanea) Cultivars by AFLP Analysis

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (5): 747-752.

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Studies on the Genetic Diversity of Some Chestnut (Castanea) Cultivars by AFLP Analysis

AI Cheng-xiang, ZHANG Li-si, WEI Hai-rong, and LIU Qing-zhong*

（Shandong Institute of Pomology, Key Laboratory for Fruit Biotechnology Breeding of Shandong, Tai' an, Shandong, 271000, China）

Received:2007-11-06 Revised:2008-01-16 Online:2008-05-25 Published:2008-05-25
Contact: LIU Qing-zhong

Abstract

Abstract:

Investigation of the genetic diversity of chestnut (Castanea) is significate in utilizing its germplasm resources and breeding new varieties. The phylogenetic relationships among 30 chestnut cultivars were analyzed by using of AFLP technique to study the genetic diversity with seven AFLP primer combinations (M+3 and E+3). Among the 962 bands detected, 852 bands were polymorphic and specific bands had been found in all tested cultivars. The high ratio of polymorphic bands (89%) observed indicated abundant genetic diversity among chestnut cultivars. The results suggested that the seven pairs of primer combinations could be used to distinguish all tested cultivars. Cluster analysis showed that the classification based on AFLP markers of most cultivars was consistent with their origin.

Key words: chestnut, cultivar, genetic diversity, AFLP

CLC Number:

S 664.2

AI Cheng-xiang;ZHANG Li-si;WEI Hai-rong;and LIU Qing-zhong. Studies on the Genetic Diversity of Some Chestnut (Castanea) Cultivars by AFLP Analysis [J]. ACTA HORTICULTURAE SINICA, 2008, 35(5): 747-752.

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Studies on the Genetic Diversity of Some Chestnut (Castanea) Cultivars by AFLP Analysis

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