Transcription Factors VlWRKY14 and VlTCP5 Respond to Exogenous CPPU and Regulate the Expression of VlIPT12 in Grape

doi:10.16420/j.issn.0513-353x.2024-0761

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (10): 2567-2581.doi: 10.16420/j.issn.0513-353x.2024-0761

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Next Articles

Transcription Factors VlWRKY14 and VlTCP5 Respond to Exogenous CPPU and Regulate the Expression of VlIPT12 in Grape

ZHAO Yaru¹^,², WANG Leilei¹^,², KONG Guanzhuo¹^,², LI Hong¹^,², ZHENG Jintao¹^,², YUE Yihan¹^,², SHI Qiaofang¹^,², ZHAO Xiaochun¹, YU Yihe¹^,²^,^*()

¹ College of Horticulture and Plant Protection，Henan University of Science and Technology，Luoyang，Henan 471023，China
² Henan Provincial Engineering Research Center on Characteristic Berry Germplasm Innovation & Utilization，Luoyang，Henan 471023，China

Received:2025-05-27 Revised:2025-06-27 Online:2025-10-25 Published:2025-10-28
Contact: YU Yihe

Abstract

Abstract:

Treatment with the cytokinin CPPU[forchlorfenuron，N-（2-chloro-4-pyridinyl）-N- phenylurea]significantly improves the fruit setting rate in grapevines，but the underlying molecular mechanisms remain unclear. To elucidate the expression of the isopentenyl transferase gene VlIPT12 after CPPU treatment and the molecular mechanism of its regulation by specific transcription factors，in this study，the VlIPT12 was cloned from‘Kyoho’grapes，and bioinformatics analysis，expression characteristics，and transcriptional regulation analysis were performed. The results showed that the full length of the VlIPT12 was 984 bp，containing a conserved ATP/ADP binding site（P-loop motif）specific to the IPT family and localizing in the nucleus. The expression level of the VlIPT12 gene was the highest in young fruits，followed by that in flowers and tendrils，and was the lowest in leaves. After the grape inflorescences were treated with CPPU，the expression level of VlIPT12 gene was significantly lower than that of the control. The VlIPT12 promoter responded to the treatments of salicylic acid and methyl jasmonate. VlWRKY14 and VlTCP5，as the key transcription factors of VlIPT12，had the same expression pattern as VlIPT12 after CPPU treatment and could bind to the key sites（P1，P2，P4）of the VlIPT12 promoter to promote its expression. In conclusion，after the grape inflorescences were treated with CPPU，the expression level of VlIPT12 decreased，and the transcription factors VlWRKY14 and VlTCP5 could bind to the key sites of the VlIPT12 promoter to positively regulate the expression of VlIPT12 in young fruits.

Key words: grape, berry set, cytokinin, VlIPT12, transcriptional regulation

ZHAO Yaru, WANG Leilei, KONG Guanzhuo, LI Hong, ZHENG Jintao, YUE Yihan, SHI Qiaofang, ZHAO Xiaochun, YU Yihe. Transcription Factors VlWRKY14 and VlTCP5 Respond to Exogenous CPPU and Regulate the Expression of VlIPT12 in Grape[J]. Acta Horticulturae Sinica, 2025, 52(10): 2567-2581.

Figures/Tables 10

References 51

[1]	Beznec A, Faccio P, Miralles D J, Abeledo L G, Oneto C D. 2021. Stress-induced expression of IPT gene in transgenic wheat reduces grain yield penalty under drought. Journal of Genetic Engineering and Biotechnology, 19 (1):67.
[2]	Böttcher C, Burbidge C A, Boss P K, Davies C. 2015. Changes in transcription of cytokinin metabolism and signalling genes in grape(Vitis vinifera L.) berries are associated with the ripening-related increase in isopentenyladenine. BMC Plant Biology, 15 (1):223.
[3]	Chen Wenwen. 2022. Functional identification of tobacco prenyltransferase gene IPT and creation of antiabiotic stress materials[M. D. Dissertation]. Chongqing: Southwest University. (in Chinese)
	陈文文. 2022. 烟草异戊烯基转移酶基因IPT的功能鉴定及创制抗非生物胁迫素材[硕士论文]. 重庆: 西南大学.
[4]	Feng Jie. 2020. Identification of rice RPL1 gene related mutants and analysis of rice IPT protein family[M. D. Dissertation]. Nanning: Guangxi University. (in Chinese)
	冯杰. 2020. 水稻RPL1基因相关突变体鉴定及水稻IPT蛋白家族分析[硕士论文]. 南宁: 广西大学.
[5]	Ghosh A, Shah M N A, Jui Z S, Saha S, Fariha K A, Islam T. 2018. Evolutionary variation and expression profiling of Isopentenyl transferase gene family in Arabidopsis thaliana L. and Oryza sativa L. Plant Gene, 15:15-27.
[6]	Guo D L, Xi F F, Yu Y H, Zhang X Y, Zhang G H, Zhong G Y. 2016. Comparative RNAseq profiling of berry development between table grape ‘Kyoho’and its early-ripening mutant‘Fengzao’. BMC Genomics, 17 (1):795.
[7]	Hsu H C, Chen W L. 2024. CPPU Improves fruit setting and growth in greenhouse-grown oriental melons(Cucumis melo L. var. makuwa Makino). HortScience, 59 (3):340-347.
[8]	Huang Wenxiao, Weng Fei, Zha Manrong, Ding Yanfeng, Wang Shaohua. 2016. Relationship of the multi-tiller phenotype of a rice mutant D12W 191 with cytokinin. Journal of Nanjing Agricultural University, 39 (5):711-721. (in Chinese)
	黄文晓, 翁飞, 査满荣, 丁艳锋, 王绍华. 2016. 水稻突变体D12W191多分蘖表型产生与细胞分裂素的关系. 南京农业大学学报, 39 (5):711-721.
[9]	Huynh-Thu V A, Irrthum A, Wehenkel L, Geurts P. 2010. Inferring regulatory networks from expression data using tree-based methods. PLOS ONE, 5 (9):e12776.
[10]	Hwang I, Sheen J, Müller B. 2012. Cytokinin signaling networks. Annual Review of Plant Biology, 63:353-380. doi: 10.1146/annurev-arplant-042811-105503 pmid: 22554243
[11]	Jia P, Wang Y, Sharif R, Ren X L, Qi G H. 2023. MdIPT1,an adenylate isopentenyltransferase coding gene from Malus domestica,is involved in branching and flowering regulation. Plant Science, 333:111730.
[12]	Jing P W, Li X F, Shi Q F, Liu H N, Pei M S, Wei T L, Guo D L, Yu Y H. 2023. Chlormequat chloride treatment inhibits grapevine stem growth via the VviRAP2.12-VviEXPA7 regulatory module. Scientia Horticulturae, 313:111891.
[13]	Krizek B A. 2009. Making bigger plants: Key regulators of final organ size. Current Opinion in Plant Biology, 12 (1):17-22. doi: 10.1016/j.pbi.2008.09.006 pmid: 18951836
[14]	Kuang J F, Wu C J, Guo Y F, Walther D, Shan W, Chen J Y, Chen L, Lu W J. 2021. Deciphering transcriptional regulators of banana fruit ripening by regulatory network analysis. Plant Biotechnology Journal, 19 (3):477-489.
[15]	Lan J Q, Wang N, Wang Y T, Jiang Y D, Yu H, Cao X F, Qin G J. 2023. Arabidopsis TCP 4 transcription factor inhibits high temperature-induced homeotic conversion of ovules. Nature Communications, 14 (1):5673.
[16]	Leshem Y Y, Wurzburger J, Grossman S, Frimer A A. 1981. Cytokinin interaction with free radical metabolism and senescence:effects on endogenous lipoxygenase and purine oxidation. Physiologia Plantarum, 53 (1):9-12.
[17]	Lewis D H, Burge G K, Hopping M E, Jameson P E. 1996. Cytokinins and fruit development in the kiwifruit(Actinidia deliciosa). Ⅱ. effects of reduced pollination and CPPU application. Physiologia Plantarum, 98 (1):187-195.
[18]	Li F, Zhang L, Ji H, Xu Z, Zhou Y, Yang S. 2020. The specific W-boxes of GAPC5 promoter bound by TaWRKY are involved in drought stress response in wheat. Plant Science, 296:110460.
[19]	Li J F, Lin T, Ren D D, Wang T, Tang Y, Wang Y W, Xu L, Zhu P K, Ma G B. 2021. Transcriptomic and metabolomic studies reveal mechanisms of effects of CPPU-mediated fruit-setting on attenuating volatile attributes of melon fruit. Agronomy, 11 (5):1007.
[20]	Li Qiaoxia, Zhang Li, Wang Yu, Huang Xiaoxia. 2019. The research progress of gibberellin on the regulation of flowering and floral organ development in plant. Chinese Journal of Cell Biology, 41 (4):746-758. (in Chinese)
	李巧峡, 张丽, 王玉, 黄小霞. 2019. 赤霉素调控植物开花及花器官发育的研究进展. 中国细胞生物学学报, 41 (4):746-758.
[21]	Lian X L, Liu S, Sikandar A, Kang Z L, Feng Y X, Jiang L Y W, Wang Y Y. 2022. The influence of 6-Benzylaminopurine(BAP)on yield responses and photosynthetic physiological indices of soybean. Kuwait Journal of Science, 50 (2):345-352.
[22]	Liu L, Wang W, Yang J, Zhang Y, Zhang G, Ma Z, Wang X. 2018. Molecular cloning of Ve promoters from Gossypium barbadense and G. hirsutum and functional analysis in verticillium wilt resistance. Plant Cell,Tissue and Organ Culture(PCTOC), 135 (3):535-544.
[23]	Lucero L E, Uberti-Manassero N G, Arce A L, Colombatti F, Alemano S G, Gonzalez D H. 2015. TCP 15 modulates cytokinin and auxin responses during gynoecium development in Arabidopsis. The Plant Journal, 84 (2):267-282.
[24]	Livak K J, Schmittgen T D. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2^{- ΔΔCT} method. Methods, 25 (4):402-408. doi: 10.1006/meth.2001.1262 pmid: 11846609
[25]	Ma Z B, Li W, Wang H P, Yu D Q. 2020. WRKY transcription factors WRKY12 and WRKY 13 interact with SPL10 to modulate age-mediated flowering. Journal of Integrative Plant Biology, 62 (11):1659-1673.
[26]	Matsuo S, Kikuchi K, Fukuda M, Honda I, Imanishi S. 2012. Roles and regulation of cytokinins in tomato fruit development. Journal of Experimental Botany, 63 (15):5569-5579. doi: 10.1093/jxb/ers207 pmid: 22865911
[27]	Maupilé L, Chaib J, Boualem A, Bendahmane A. 2024. Parthenocarpy,a pollination-independent fruit set mechanism to ensure yield stability. Trends in Plant Science, 29 (11):1254-1265.
[28]	Miyawaki K, Tarkowski P, Matsumoto-Kitano M, Kato T, Sato S, Tarkowska D, Tabata S, Sandberg G, Kakimoto T. 2006. Roles of Arabidopsis ATP/ADP isopentenyltransferases and tRNA isopentenyltransferases in cytokinin biosynthesis. Proceedings of the National Academy of Sciences, 103 (44):16598-16603.
[29]	Moore S, Jervis G, Topping J F, Chen C, Liu J, Lindsey K. 2024. A predictive model for ethylene-mediated auxin and cytokinin patterning in the Arabidopsis root. Plant Communications, 15 (7):100886.
[30]	Nguyen H N, Lai N, Kisiala A B, Emery R J N. 2021. Isopentenyltransferases as master regulators of crop performance:their function,manipulation,and genetic potential for stress adaptation and yield improvement. Plant Biotechnology Journal, 19 (7):1297-1313. doi: 10.1111/pbi.13603 pmid: 33934489
[31]	Okatan V. 2020. Antioxidant properties and phenolic profile of the most widely appreciated cultivated berry species:a comparative study. Folia Horticulturae, 32 (1):79-85.
[32]	O’Keefe D, Song J C, Jameson P. 2010. Isopentenyl transferase and cytokinin oxidase/dehydrogenase gene family members are differentially expressed during pod and seed development in rapid-cycling brassica. Journal of Plant Growth Regulation, 30:92-99.
[33]	Quinet M, Buyens C, Dobrev P I, Motyka V, Jacquemart A L. 2019. Hormonal regulation of early fruit development in european pear(Pyrus communis cv.‘Conference’). Horticulturae, 5 (1):9.
[34]	Sovak M. 2001. Grape extract,resveratrol,and its analogs:a review. Journal of Medicinal Food, 4 (2):93-105.
[35]	Steiner E, Yanai O, Efrini I, Ori N, Eshed Y, Weiss D. 2012. Class I TCPs modulate cytokinin-induced branching and meristematic activity in tomato. Plant Signaling & Behavior, 7 (7):807-810.
[36]	Su Hang, Zhang Peng, Li Hui, Wang Lufang, Jin Fen. 2017. Effect of plant growth regulators in common use on fruit quality in China. Quality and Safety of Agro-Products,(2):44-48. (in Chinese)
	苏杭, 张鹏, 李慧, 王璐芳, 金芬. 2017. 我国常用植物生长调节剂对水果品质影响研究. 农产品质量与安全,(2):44-48.
[37]	Sun Y D, Yue Y H, Li X F, Li X Q, Shi Q F, Yu Y H. 2024. Transcription factor VviWOX13C regulates fruit set by directly activating VviEXPA37/38/39 in grape(Vitis vinifera L). Plant Cell Reports, 43 (1):19.
[38]	Vlot A C, Dempsey D A, Klessig D F. 2009. Salicylic acid,a multifaceted hormone to combat disease. Annual Review of Phytopathology, 47 (1):177-206.
[39]	Wang L L, Shi Q F, Jing P W, Wang R X, Zhang H M, Liu Y T, Li C Y, Shi T Z, Zhang L X, Yu Y H. 2024. VlMYB4 and VlCDF 3 co-targeted the VlLOG11 promoter to regulate fruit setting in grape(Vitis vinifera L). Plant Cell Reports, 43 (8):194. doi: 10.1007/s00299-024-03279-8 pmid: 39008131
[40]	Wei T, Guo D, Liu J. 2021. PtrMYB3,a R2R3-MYB transcription factor from poncirus trifoliata,negatively regulates salt tolerance and hydrogen peroxide scavenging. Antioxidants, 10 (9):1388.
[41]	Xiong R Q, Peng Z H, Zhou H, Xue G X, He A, Yao X, Weng W F, Wu W J, Ma C, Bai Q, Ruan J J. 2024. Genome-wide identification,structural characterization and gene expression analysis of the WRKY transcription factor family in pea(Pisum sativum L.). BMC Plant Biology, 24 (1):113.
[42]	Xu C C, Wang X, Wu Y, Gao J, Zhang P, Zhao Y T, Liu X L, Wang P, Huang S B. 2024a. Molecular mechanisms underlying low temperature inhibition of grain filling in maize(Zea mays L.):coordinationof growth and cold responses. The Plant Journal, 119 (2):982-997.
[43]	Xu J, Xing X J, Tian Y S, Peng R H, Xue Y, Zhao W, Yao Q H. 2015. Transgenic arabidopsis plants expressing tomato glutathione S-transferase showed enhanced resistance to salt and drought stress. PLoS ONE, 10 (9):e0136960.
[44]	Xu R, Chong L, Zhu Y F. 2024b. Mediator kinase subunit CDK 8 phosphorylates transcription factor TCP15 during tomato pollen development. Plant Physiology, 195 (1):865-878.
[45]	Yadav P, Yadav S K, Singh M, Singh M P, Chinnusamy V. 2024. Genome wide identification and characterization of Isopentenyl transferase(IPT) gene family associated with cytokinin synthesis in rice. Plant Physiology Reports, 29 (1):1-19.
[46]	Yuan Qiaohui. 2023. Preliminary study on the regulation of cytokinin on the quality of straw berry fruit[M. D. Dissertation]. Nanjing: Nanjing Agriculture University. (in Chinese)
	原乔慧. 2023. 细胞分裂素对草莓果实品质调控作用初探[硕士论文]. 南京: 南京农业大学.
[47]	Zabadal T J, Bukovac M J. 2006. Effect of CPPU on fruit development of selected seedless and seeded grape cultivars. HortScience, 41 (1):154-157.
[48]	Zhang L P, Li M, Fu J Y, Huang X Q, Yan P, Ge S, Li Z Z, Bai P X, Zhang L, Han W Y, Li X. 2022. Genome-wide identification and expression analysis of Isopentenyl transferase family genes during development and resistance to abiotic stresses in tea plant(Camellia sinensis). Plants, 11 (17):2243.
[49]	Zhang Yanyi, Jia Haifeng, Zhao Fanggui, Wang Baojü, Li Xiaopeng, Wang Chen. 2014. Effect of gibberellin treatment on photosynthesis and chlorophyll fluorescence characteristics of summer black grape leaf. Jiangsu Journal of Agricultural Sciences, 30 (6):1472-1479. (in Chinese)
	张演义, 贾海锋, 赵方贵, 王保菊, 李晓鹏, 王晨. 2014. 赤霉素处理夏黑葡萄果穗对叶片光合及荧光特性的影响. 江苏农业学报, 30 (6):1472-1479.
[50]	Zhao Keke, Cui Lulu, Li Chunxiu, Dang Jiangbo, Liang Guolu, Xiang Suqiong. 2021. Research advances on parthenocarpy in citrus. Acta Horticulturae Sinica, 48 (4):811-824. (in Chinese) doi: 10.16420/j.issn.0513-353x.2020-0881 URL
	赵科科, 崔璐璐, 李春秀, 党江波, 梁国鲁, 向素琼. 2021. 柑橘单性结实研究进展. 园艺学报, 48 (4):811-824. doi: 10.16420/j.issn.0513-353x.2020-0881 URL
[51]	Zhou C, Lin Q, Lan J, Zhang T, Liu X, Miao R, Mou C, Nguyen T, Wang J, Zhang Xiao. 2020. WRKY transcription factor OsWRKY29 represses seed dormancy in rice by weakening abscisic acid response. Frontiers in Plant Science, 11:691.

用途 Usage	引物名称 Primer name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
基因克隆 Gene clone	FL-VlIPT12	GGACCTCACCATGGATGTCTCC	CTAGTGGGTTGCTGCTAGGGCA
	Pro-VlIPT12	GGGCAATTTGGGTCTGAAGTGCT	GTAGAATCAGAGATGCAGCAACCG
	FL-VlWRKY14	GTGATCCTTCAGCTGGTTTCCCA	CCAAGTTTATGCCAGGACCCTTG
	FL-VlTCP5	CCTGCACGCTGGACCATCAAGAA	CCATGGCGAAGCATACTATCTGGC
GUS活性验证 GUS activity verification	GUS-ProVlIPT12	TGGGCCCGGCGCGCCAAGCTTG GGCAATTTGGGTCTGAAGTGCT	GGTGGACTCCTCTTAGAATTCGT AGAATCAGAGATGCAGCAACCG
亚细胞定位 Subcellular localization	YFP-VlIPT12	ATGGGATCTACTAGTGAATTCGGACCTCACCATGGATGTCTCC	GGGGGTACCGTCGACGGATCCCTAGTGGGTTGCTGCTAGGGCA
DLR验证 DLR verification	LUC-VlIPT12	GTCGACGGTATCGATAAGCTTGGACCTCACCATGGATGTCTCC	CGCTCTAGAACTAGTGGATCC CTAGTGGGTTGCTGCTAGGGCA
Y1H验证 Y1H verification	TCP5-IPT12-TFBD1	GAGGAACCATTCTTCAAAGTGGG	CCCAAATTGCCCATGATTATGGC
	TCP5-IPT12-TFBD2	GATTTGATACGAGTCATCTGG	ACCGACATCTGATTCCTATCC
	TCP5-IPT12-TFBD3	GGATATCAACATCACCCTGCCC	TTAGCTTGGAGTGGTGGCCC
	WRKY14-IPT12-TFBD1	GTACTATTGACCTTACGCGC	GCCTACGAGTAAATGATAGTC
	WRKY14-IPT12-TFBD2	CTTAATAGATAGAAACCCTCCC	CTACCAATCATATGTCTCAAGA
	WRKY14-IPT12-TFBD3	GGCCACCACTCCAAGCTAAGC	GGGTCAAGGACCTCTGCTCAAC
	AD-EcoRI-QWRKY14	GCCATGGAGGCCAGTGAATTCGTGATCCTTCAGCTGGTTTCCCA	CAGCTCGAGCTCGATGGATCC CCAAGTTTATGCCAGGACCCTTG
	AD-EcoRI-QTCP5	GCCATGGAGGCCAGTGAATTCCCTGCACGCTGGACCATCAAGAA	CAGCTCGAGCTCGATGGATCCCCATGGCGAAGCATACTATCTGGC

用途 Usage	引物名称 Primer name	正向引物（5′-3′） Forward primer	反向引物（5′-3′） Reverse primer
基因克隆 Gene clone	FL-VlIPT12	GGACCTCACCATGGATGTCTCC	CTAGTGGGTTGCTGCTAGGGCA
	Pro-VlIPT12	GGGCAATTTGGGTCTGAAGTGCT	GTAGAATCAGAGATGCAGCAACCG
	FL-VlWRKY14	GTGATCCTTCAGCTGGTTTCCCA	CCAAGTTTATGCCAGGACCCTTG
	FL-VlTCP5	CCTGCACGCTGGACCATCAAGAA	CCATGGCGAAGCATACTATCTGGC
GUS活性验证 GUS activity verification	GUS-ProVlIPT12	TGGGCCCGGCGCGCCAAGCTTG GGCAATTTGGGTCTGAAGTGCT	GGTGGACTCCTCTTAGAATTCGT AGAATCAGAGATGCAGCAACCG
亚细胞定位 Subcellular localization	YFP-VlIPT12	ATGGGATCTACTAGTGAATTCGGACCTCACCATGGATGTCTCC	GGGGGTACCGTCGACGGATCCCTAGTGGGTTGCTGCTAGGGCA
DLR验证 DLR verification	LUC-VlIPT12	GTCGACGGTATCGATAAGCTTGGACCTCACCATGGATGTCTCC	CGCTCTAGAACTAGTGGATCC CTAGTGGGTTGCTGCTAGGGCA
Y1H验证 Y1H verification	TCP5-IPT12-TFBD1	GAGGAACCATTCTTCAAAGTGGG	CCCAAATTGCCCATGATTATGGC
	TCP5-IPT12-TFBD2	GATTTGATACGAGTCATCTGG	ACCGACATCTGATTCCTATCC
	TCP5-IPT12-TFBD3	GGATATCAACATCACCCTGCCC	TTAGCTTGGAGTGGTGGCCC
	WRKY14-IPT12-TFBD1	GTACTATTGACCTTACGCGC	GCCTACGAGTAAATGATAGTC
	WRKY14-IPT12-TFBD2	CTTAATAGATAGAAACCCTCCC	CTACCAATCATATGTCTCAAGA
	WRKY14-IPT12-TFBD3	GGCCACCACTCCAAGCTAAGC	GGGTCAAGGACCTCTGCTCAAC
	AD-EcoRI-QWRKY14	GCCATGGAGGCCAGTGAATTCGTGATCCTTCAGCTGGTTTCCCA	CAGCTCGAGCTCGATGGATCC CCAAGTTTATGCCAGGACCCTTG
	AD-EcoRI-QTCP5	GCCATGGAGGCCAGTGAATTCCCTGCACGCTGGACCATCAAGAA	CAGCTCGAGCTCGATGGATCCCCATGGCGAAGCATACTATCTGGC

顺式作用元件 cis-Acting elements	序列 Sequence	功能 Function	元件数量 Number
TGACG-motif	TGACG	茉莉酸甲酯响应元件 MeJA responsive element	1
GT1-motif	GGTTAA	光调控元件 Light responsive element	2
GATA-motif	GATAGGG/GATAGGA	光调控元件 Light responsive element	2
Sp1	GGGCGG	光调控元件 Light responsive element	1
TCT-motif	TCTTAC	光调控元件 Light responsive element	1
TGA-element	AACGAC	生长素响应元件 Auxin responsive element	1
MBS	CAACTG	参与干旱诱导的调控元件Drought-inducibility responsive element	2
TCA-element	CCATCTTTTT	水杨酸响应元件 Salicylic acid responsive element	1
CGTCA-motif	CGTCA	茉莉酸甲酯响应元件 MeJA responsive element	1
W-box	TGACC	未知 Unknown	2
CAT-box	GCCACT	分生组织表达相关调控元件 cis-Acting regulatory element related to meristem expression	2
GC-motif	CCCCCG	参与缺氧特异性诱导的增强子元件 Enhancer-like element involved in anoxic specific inducibility	1
ARE	AAACCA	参与厌氧特异性诱导的增强子元件 cis-Acting regulatory element essential for the anaerobic induction	1
MYB	CAACCA/TAACCA/CAACTG	未知 Unknown	4
AAGAA-motif	GAAAGAA	未知 Unknown	1
TATA-box	TATA	核心启动元件 Core promoter element	36
CAAT-box	CAAT/CCAAT/CAAAT	启动子区和增强子区常见元件 Common cis-acting element in promoter and enhancer regions	42

顺式作用元件 cis-Acting elements	序列 Sequence	功能 Function	元件数量 Number
TGACG-motif	TGACG	茉莉酸甲酯响应元件 MeJA responsive element	1
GT1-motif	GGTTAA	光调控元件 Light responsive element	2
GATA-motif	GATAGGG/GATAGGA	光调控元件 Light responsive element	2
Sp1	GGGCGG	光调控元件 Light responsive element	1
TCT-motif	TCTTAC	光调控元件 Light responsive element	1
TGA-element	AACGAC	生长素响应元件 Auxin responsive element	1
MBS	CAACTG	参与干旱诱导的调控元件Drought-inducibility responsive element	2
TCA-element	CCATCTTTTT	水杨酸响应元件 Salicylic acid responsive element	1
CGTCA-motif	CGTCA	茉莉酸甲酯响应元件 MeJA responsive element	1
W-box	TGACC	未知 Unknown	2
CAT-box	GCCACT	分生组织表达相关调控元件 cis-Acting regulatory element related to meristem expression	2
GC-motif	CCCCCG	参与缺氧特异性诱导的增强子元件 Enhancer-like element involved in anoxic specific inducibility	1
ARE	AAACCA	参与厌氧特异性诱导的增强子元件 cis-Acting regulatory element essential for the anaerobic induction	1
MYB	CAACCA/TAACCA/CAACTG	未知 Unknown	4
AAGAA-motif	GAAAGAA	未知 Unknown	1
TATA-box	TATA	核心启动元件 Core promoter element	36
CAAT-box	CAAT/CCAAT/CAAAT	启动子区和增强子区常见元件 Common cis-acting element in promoter and enhancer regions	42

Transcription Factors VlWRKY14 and VlTCP5 Respond to Exogenous CPPU and Regulate the Expression of VlIPT12 in Grape

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