Analysis of Floral Volatile Components Among Different Bearded Iris Cultivars Using GC-IMS

doi:10.16420/j.issn.0513-353x.2024-0379

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (6): 1619-1632.doi: 10.16420/j.issn.0513-353x.2024-0379

• Cultivation?Physiology & Biochemistry • Previous Articles Next Articles

Analysis of Floral Volatile Components Among Different Bearded Iris Cultivars Using GC-IMS

SUN Junming¹, LI Xiaoyun¹, ZHU Ying², LOU Qian¹, and CHEN Hongwu¹^,^*()

¹ College of Horticulture，Northwest A & F University，Yangling，Shannxi 712100，China
² Beijing Botanical Garden，Beijing 100093，China

Received:2024-11-25 Revised:2025-02-17 Online:2025-06-20 Published:2025-06-20
Contact: and CHEN Hongwu

Abstract

Abstract:

To identify the composition of floral volatile components and characteristic aromas of Iris × barbata，the flower volatile compounds of 16 bearded iris cultivars in full bloom were analyzed using gas chromatography-ion mobility spectrometry（GC-IMS）A total of 55 volatile compounds were identified，including 21 fatty acid derivatives，17 terpenoids，3 benzenoids/phenylpropanoids，1 miscellaneous cyclic compound and 13 unidentified compounds. Terpenoids dominated all 16 bearded iris cultivars，followed by fatty acid derivatives. The composition of volatile compounds varied among the cultivars. Principal component analysis（PCA）indicated that α-pinene，linalool，β-pinene，limonene，myrcene，methyl 2-methylbutyrate，2-methylbutanol，methyl isovalerate，hexanol，and 3-methyl-2-butenal were the major volatile compounds in bearded iris. Analysis of relative odor activity values（ROAV）showed that methyl 2-methylbutyrate，2-octanone，myrcene and α-pinene were the main aromacontributors to the fruity and spicy aromas of the 16 bearded iris cultivars，and linalool contributed to the floral aroma of some varieties.

Key words: Iris × barbata, GC-IMS, volatile components, floral aroma

SUN Junming, LI Xiaoyun, ZHU Ying, LOU Qian, and CHEN Hongwu. Analysis of Floral Volatile Components Among Different Bearded Iris Cultivars Using GC-IMS[J]. Acta Horticulturae Sinica, 2025, 52(6): 1619-1632.

Figures/Tables 10

References 38

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化合物Compounds	相对含量/% Relative content
化合物Compounds	C1	C2	C3	C4	C5	C6	C7	C8
α-蒎烯α-Pinene	18.28 ± 0.30	16.71 ± 0.52	21.12 ± 0.26	19.17 ± 0.60	19.04 ± 0.38	19.85 ± 0.33	20.88 ± 0.32	15.72 ± 0.25
芳樟醇Linalool	11.79 ± 0.10	14.23 ± 0.32	15.35 ± 0.29	12.71 ± 1.00	8.25 ± 0.64	—	21.63 ± 1.11	17.41 ± 1.74
β-蒎烯β-Pinene	9.66 ± 0.11	8.86 ± 0.20	11.31 ± 0.26	10.22 ± 0.87	9.72 ± 0.15	11.17 ± 0.03	10.14 ± 0.52	6.74 ± 0.39
双戊烯Limonene	9.29 ± 0.23	8.37 ± 0.13	11.09 ± 0.26	9.34 ± 0.95	8.25 ± 0.21	10.90 ± 0.05	10.08 ± 0.65	5.65 ± 0.22
月桂烯Myrcene	6.61 ± 0.11	6.63 ± 0.26	8.44 ± 0.20	6.93 ± 0.75	6.50 ± 0.35	7.90 ± 0.07	7.27 ± 0.64	4.24 ± 0.12
2-甲基丁酸甲酯 Methyl 2-methylbutyrate	1.57 ± 0.01	1.72 ± 0.17	1.99 ± 0.09	4.60 ± 0.43	7.29 ± 0.16	8.68 ± 0.11	2.66 ± 0.11	9.17 ± 0.79
2-甲基丁醇 2-Methylbutanol	2.97 ± 0.10	2.65 ± 0.17	2.36 ± 0.13	2.50 ± 0.88	2.59 ± 0.21	2.02 ± 0.40	2.53 ± 0.26	3.43 ± 0.16
正己醇Hexanol	2.86 ± 0.26	2.94 ± 0.58	2.26 ± 0.18	1.94 ± 0.05	2.72 ± 0.13	2.36 ± 0.44	0.45 ± 0.10	4.90 ± 0.92
3-甲基-2-丁烯醛 3-Methyl-2-butenal	1.93 ± 0.31	1.45 ± 0.35	3.76 ± 0.44	3.08 ± 0.04	0.85 ± 0.06	0.74 ± 0.02	1.58 ± 0.24	4.84 ± 0.66
化合物Compounds	相对含量/% Relative content
化合物Compounds	C9	C10	C11	C12	C13	C14	C15	C16
α-蒎烯α-Pinene	1.76 ± 0.07	21.75 ± 0.28	21.42 ± 0.08	0.65 ± 0.03	17.24 ± 0.23	18.09 ± 0.92	25.07 ± 0.31	1.41 ± 0.20
芳樟醇Linalool	22.53 ± 0.32	7.37 ± 0.61	1.45 ± 0.20	23.10 ± 1.79	11.94 ± 0.70	4.73 ± 1.22	—	—
β-蒎烯β-Pinene	—	9.17 ± 0.13	9.88 ± 0.06	—	6.36 ± 0.08	5.11 ± 0.42	7.45 ± 0.51	3.08 ± 0.49
双戊烯Limonene	1.25 ± 0.03	8.47 ± 0.10	8.44 ± 0.17	1.22 ± 0.04	5.43 ± 0.08	5.69 ± 0.42	6.77 ± 0.44	—
月桂烯Myrcene	0.75 ± 0.01	6.48 ± 0.06	6.88 ± 0.18	0.60 ± 0.01	4.94 ± 0.09	4.90 ± 0.27	4.90 ± 0.33	2.23 ± 0.30
2-甲基丁酸甲酯 Methyl 2-methylbutyrate	3.41 ± 0.03	4.93 ± 0.15	7.08 ± 0.47	4.41 ± 0.05	9.47 ± 0.52	7.97 ± 0.99	5.07 ± 0.40	5.38 ± 0.45
2-甲基丁醇 2-Methylbutanol	8.69 ± 0.05	3.33 ± 0.09	4.49 ± 0.14	7.25 ± 0.41	2.69 ± 0.52	6.49 ± 1.08	7.29 ± 0.29	7.18 ± 0.88
异戊酸甲酯 Methyl isovalerate	4.18 ± 0.20	2.71 ± 0.08	4.67 ± 0.24	2.55 ± 0.25	5.88 ± 0.36	4.28 ± 1.47	1.19 ± 0.04	13.39 ± 7.02
正己醇Hexanol	5.74 ± 0.10	5.01 ± 0.21	3.55 ± 0.31	9.51 ± 0.49	1.06 ± 0.02	4.89 ± 0.61	2.22 ± 0.05	3.70 ± 1.28
3-甲基-2-丁烯醛 3-Methyl-2-butenal	1.54 ± 0.03	1.74 ± 0.20	2.79 ± 0.20	2.61 ± 0.36	3.59 ± 0.18	7.64 ± 0.63	1.89 ± 0.03	11.46 ± 3.75

化合物Compounds	相对含量/% Relative content
化合物Compounds	C1	C2	C3	C4	C5	C6	C7	C8
α-蒎烯α-Pinene	18.28 ± 0.30	16.71 ± 0.52	21.12 ± 0.26	19.17 ± 0.60	19.04 ± 0.38	19.85 ± 0.33	20.88 ± 0.32	15.72 ± 0.25
芳樟醇Linalool	11.79 ± 0.10	14.23 ± 0.32	15.35 ± 0.29	12.71 ± 1.00	8.25 ± 0.64	—	21.63 ± 1.11	17.41 ± 1.74
β-蒎烯β-Pinene	9.66 ± 0.11	8.86 ± 0.20	11.31 ± 0.26	10.22 ± 0.87	9.72 ± 0.15	11.17 ± 0.03	10.14 ± 0.52	6.74 ± 0.39
双戊烯Limonene	9.29 ± 0.23	8.37 ± 0.13	11.09 ± 0.26	9.34 ± 0.95	8.25 ± 0.21	10.90 ± 0.05	10.08 ± 0.65	5.65 ± 0.22
月桂烯Myrcene	6.61 ± 0.11	6.63 ± 0.26	8.44 ± 0.20	6.93 ± 0.75	6.50 ± 0.35	7.90 ± 0.07	7.27 ± 0.64	4.24 ± 0.12
2-甲基丁酸甲酯 Methyl 2-methylbutyrate	1.57 ± 0.01	1.72 ± 0.17	1.99 ± 0.09	4.60 ± 0.43	7.29 ± 0.16	8.68 ± 0.11	2.66 ± 0.11	9.17 ± 0.79
2-甲基丁醇 2-Methylbutanol	2.97 ± 0.10	2.65 ± 0.17	2.36 ± 0.13	2.50 ± 0.88	2.59 ± 0.21	2.02 ± 0.40	2.53 ± 0.26	3.43 ± 0.16
正己醇Hexanol	2.86 ± 0.26	2.94 ± 0.58	2.26 ± 0.18	1.94 ± 0.05	2.72 ± 0.13	2.36 ± 0.44	0.45 ± 0.10	4.90 ± 0.92
3-甲基-2-丁烯醛 3-Methyl-2-butenal	1.93 ± 0.31	1.45 ± 0.35	3.76 ± 0.44	3.08 ± 0.04	0.85 ± 0.06	0.74 ± 0.02	1.58 ± 0.24	4.84 ± 0.66
化合物Compounds	相对含量/% Relative content
化合物Compounds	C9	C10	C11	C12	C13	C14	C15	C16
α-蒎烯α-Pinene	1.76 ± 0.07	21.75 ± 0.28	21.42 ± 0.08	0.65 ± 0.03	17.24 ± 0.23	18.09 ± 0.92	25.07 ± 0.31	1.41 ± 0.20
芳樟醇Linalool	22.53 ± 0.32	7.37 ± 0.61	1.45 ± 0.20	23.10 ± 1.79	11.94 ± 0.70	4.73 ± 1.22	—	—
β-蒎烯β-Pinene	—	9.17 ± 0.13	9.88 ± 0.06	—	6.36 ± 0.08	5.11 ± 0.42	7.45 ± 0.51	3.08 ± 0.49
双戊烯Limonene	1.25 ± 0.03	8.47 ± 0.10	8.44 ± 0.17	1.22 ± 0.04	5.43 ± 0.08	5.69 ± 0.42	6.77 ± 0.44	—
月桂烯Myrcene	0.75 ± 0.01	6.48 ± 0.06	6.88 ± 0.18	0.60 ± 0.01	4.94 ± 0.09	4.90 ± 0.27	4.90 ± 0.33	2.23 ± 0.30
2-甲基丁酸甲酯 Methyl 2-methylbutyrate	3.41 ± 0.03	4.93 ± 0.15	7.08 ± 0.47	4.41 ± 0.05	9.47 ± 0.52	7.97 ± 0.99	5.07 ± 0.40	5.38 ± 0.45
2-甲基丁醇 2-Methylbutanol	8.69 ± 0.05	3.33 ± 0.09	4.49 ± 0.14	7.25 ± 0.41	2.69 ± 0.52	6.49 ± 1.08	7.29 ± 0.29	7.18 ± 0.88
异戊酸甲酯 Methyl isovalerate	4.18 ± 0.20	2.71 ± 0.08	4.67 ± 0.24	2.55 ± 0.25	5.88 ± 0.36	4.28 ± 1.47	1.19 ± 0.04	13.39 ± 7.02
正己醇Hexanol	5.74 ± 0.10	5.01 ± 0.21	3.55 ± 0.31	9.51 ± 0.49	1.06 ± 0.02	4.89 ± 0.61	2.22 ± 0.05	3.70 ± 1.28
3-甲基-2-丁烯醛 3-Methyl-2-butenal	1.54 ± 0.03	1.74 ± 0.20	2.79 ± 0.20	2.61 ± 0.36	3.59 ± 0.18	7.64 ± 0.63	1.89 ± 0.03	11.46 ± 3.75

Analysis of Floral Volatile Components Among Different Bearded Iris Cultivars Using GC-IMS

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