Intercropping of Allium，Mustard，and Alfalfa on the Growth Performance and Soil Environment of Replant Soil-Cultivated Peach Plants

doi:10.16420/j.issn.0513-353x.2023-0985

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (1): 185-199.doi: 10.16420/j.issn.0513-353x.2023-0985

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Intercropping of Allium，Mustard，and Alfalfa on the Growth Performance and Soil Environment of Replant Soil-Cultivated Peach Plants

DU Haowei, LIAO Wei, HUANG Xue, YANG Jinzhi, SHEN Wanqi, ZHANG Dongmei, LIU Junwei^*(), LI Guohuai

National Key Lab for Germplasm Innovation and Utilization of Horticultural Crops，College of Horticulture and Forestry Sciences，Huazhong Agricultural University，Wuhan 430070，China

Received:2024-06-17 Revised:2024-10-24 Online:2025-01-25 Published:2025-01-19
Contact: LIU Junwei

Abstract

Abstract:

To explore the feasibility of using intercropping to mitigate peach replant problem，peach seedlings grown in replanted soil were intercropped with onion，shallot，garlic，Chinese leek，mustard，and alfalfa for 90 days. After this period，the growth characteristics and physiological-biochemical indices of peach seedlings were measured，alongside changes in soil physicochemical properties，enzyme activity and microbial community. Results showed that，except for mustard，intercropping with all other crops improved peach seedlings growth，with garlic having the most significant effect. Garlic intercropping significantly increased seedlings height，stem diameter，leaf number，leaf area，chlorophyll content，aboveground and root biomass，and promoted root growth. Additionally，it increased soil organic matter， available potassium，soil urease，catalase and invertase activity，as well as the abundance of beneficial bacteria like Streptomyces and fungi like Clitopilus，while reducing the abundance of predicted plant pathogenic fungi. In summary，intercropping suitable crops can alleviate peach replant problem by regulating soil microbial community structure，optimizing replanted soil environment，and promoting plant growth. Garlic，in particular，showed the most prominent effects. Thus，intercropping or rotating garlic in peach orchards or in the renewal of old orchards holds potential as an effective strategy for alleviating peach replant problem.

Key words: Prunus persica, replant problem, intercropping, rhizosphere microbiota, soil enzyme activity, soil physiochemical property

DU Haowei, LIAO Wei, HUANG Xue, YANG Jinzhi, SHEN Wanqi, ZHANG Dongmei, LIU Junwei, LI Guohuai. Intercropping of Allium，Mustard，and Alfalfa on the Growth Performance and Soil Environment of Replant Soil-Cultivated Peach Plants[J]. Acta Horticulturae Sinica, 2025, 52(1): 185-199.

Figures/Tables 9

Fig. 1 Growth performance of potted peach seedlings in replanted soil under mixed planting of different crops

Fig. 2 Growth performance of replant soil-potted peach seedlings under different mixed cropping treatments Ac：Allium cepa；Af：A. fistulosum；At：A. tuberosum；As：A. sativum；Bj：Brassica juncea；Ms：Medicago sativa. Different letters indicate significant differences as conducted by Duncan’s multiple range test at P < 0.05. The same below

Fig. 3 Root growth performance of replant soil-potted peach seedling under different mixed cropping treatments

Fig. 4 SPAD and chlorophyll content of peach leaves grown in peach replanted soil under different mixed cropping treatments

Table 1 Physicochemical properties of peach replanted soil under different mixed cropping treatments with peach seedlings

作物 Crop	有机质/% Ogranic matter	mg · kg^-1						pH
作物 Crop	有机质/% Ogranic matter	碱解氮 Alkali- hydrolyzable N	速效磷 Available P	速效钾 Available K	有效钙 Available Ca	有效镁 Available Mg	有效钠 Available Na	pH
对照Control	4.05 ± 0.11 b	71.93 ± 3.86 b	19.20 ± 0.92 a	98.58 ± 5.86 b	2 602.74 ± 66.78 b	546.72 ± 17.42 b	120.09 ± 1.22 c	5.90 ± 0.09 a
芥菜Brassica juncea	3.89 ± 0.04 b	80.78 ± 3.75 a	16.27 ±0.72 b	103.90 ± 3.29 b	2 894.05 ± 146.79 a	632.42 ± 25.21 a	128.86 ± 2.19 b	5.77 ± 0.07 b
苜蓿Medicago sativa	3.62 ± 0.03 c	74.46 ± 2.48 ab	17.62 ±0.62 ab	120.49 ± 3.11 a	2 702.73 ± 62.20 ab	617.22 ± 17.11 a	137.67 ± 3.16 a	5.72 ± 0.02 b
大蒜Allium sativum	4.24 ± 0.16 a	74.48 ± 2.77 ab	17.64 ±1.13 ab	118.94 ± 5.80 a	2 246.67 ± 112.66 c	532.36 ± 28.34 b	122.44 ± 2.81 c	5.81 ± 0.07 ab

Fig. 5 Enzyme activities of peach replanted soil under different mixed cropping treatments with peach seedlings

Fig. 6 Relative abundance of rhizosphere fungal（A，C）and bacterial（B，D-F）communities of peach plants grown in replanted soil under different mixed cropping treatments Asterisks indicate significant differences between treatments and the control as conducted by Tukey test at * P < 0.05，** P < 0.01

Fig. 7 Redundancy analysis of soil physiochemical properties and microbial community under different mixed copping treatments AN：Available nitrogen，AP：Available phosphate，AK：Available potassium；Aca：Available calcium；SOM：Soil organic matter； SC：Soil catalase，SU：Soil urease；SNI：Soil neutral invertase. The dots of different colors represent different sample points. The quadrant of each environmental factor represents the positive and negative correlation between the environmental factor and the sorting axis. The length of the arrow represents the degree of correlation（the longer the arrow，the greater the correlation，and vice versa）

Fig. 8 Functional prediction analysis of soil fungi using FUNGuild（A）and bacteria using PICRUSt1（B）under different mixed planting treatments

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Intercropping of Allium，Mustard，and Alfalfa on the Growth Performance and Soil Environment of Replant Soil-Cultivated Peach Plants

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