Prokaryotic Expression of MxIrt1 Genes Segment and the Prepara tion of ItsPolyclonal Antibody

ACTA HORTICULTURAE SINICA ›› 2009, Vol. 36 ›› Issue (10): 1411-1416.

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Prokaryotic Expression of MxIrt1 Genes Segment and the Prepara tion of ItsPolyclonal Antibody

SUN Chun-yu^1,2;WANG Yi¹;KONG Jin¹;XU Xue-feng¹;L I Tian-zhong¹;HAN Zhen-hai^1*

(¹Institute of Horticultural Plants, China AgriculturalUniversity, Beijing 100193, China; ²College of Life Sciences, Jilin Agricultural University,Changchun130118, China)

Received:2009-03-02 Revised:2009-08-29 Online:2009-10-25 Published:2009-10-25
Contact: HAN Zhen-hai

Abstract

Abstract: MxIrt1 is a iron transport p rotein gene from M alus xiaojinensis. To study the functional characteristic of MxIrt1, the recombinant plasmid pGEX-MxIrt1 was constructed with 162 bp segments between 3 rd and 4 th TMR (Transmembrane region) , following the expression in E.coli with high efficiency, purification using Glutathione Sepharose 4B, the recombinant GST-MxIRT1 protein was obtained. ELISA assay detected the reactivity of the polycolonal antibody andWestern blot shows that specific band was corresponding to the expectation. The recombinant GST-MxIRT1 protein can also be used for immunohistochemistry.

Key words: Malus, Malus xiaojinensis, MxIrt1, specific segment, prokaryotic expression, polyclonal antibody

CLC Number:

SUN Chun-yu;WANG Yi;KONG Jin;XU Xue-feng;L I Tian-zhong;HAN Zhen-hai. Prokaryotic Expression of MxIrt1 Genes Segment and the Prepara tion of ItsPolyclonal Antibody[J]. ACTA HORTICULTURAE SINICA, 2009, 36(10): 1411-1416.

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Prokaryotic Expression of MxIrt1 Genes Segment and the Prepara tion of ItsPolyclonal Antibody

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